Department of Biological Sciences, University of Bergen, P.O. Box 7803, N-5006, Bergen, Norway.
Department of Mathematics, University of Bergen, P.O. Box 7803, N-5006, Bergen, Norway; Department of Psychiatry and Psychotherapy, University Regensburg, Regensburg, Germany.
Fish Shellfish Immunol. 2019 Dec;95:650-658. doi: 10.1016/j.fsi.2019.11.016. Epub 2019 Nov 6.
Two monovalent vaccines against pasteurellosis were developed and tested for efficacy using a previously established bath challenge model. High levels of specific antibodies were detected following vaccination. While the vaccine efficacy trial indicated that some level of protection was obtained, high mortality was still observed. qPCR analysis of head kidney tissues from surviving fish post challenge showed no difference in bacterial numbers in vaccinated and non-vaccinated fish. Clinical symptoms observed in moribund and diseased fish included white spots on the skin and around the eyes, frayed fins and redness around the mouth and fin bases. Despite production of specific antibodies, the protection against experimental challenge was relatively weak. A reason for this could potentially be that the specific antibodies produced are not alone enough to provide complete protection against pasteurellosis in lumpsuckers. Confocal and scanning electron microscopy of head kidney leucocytes exposed to Pasteurella sp. in vitro gave indications of the interactions between the pathogen and leucocytes. The results indicate that parts of the immune system other than humoral antibodies could be important for protection against pasteurellosis. Our combined results highlight the need for further work on host-pathogen interaction between Pasteurella sp. and lumpsuckers.
两种单价巴氏杆菌疫苗已被开发出来，并使用先前建立的浴挑战模型对其功效进行了测试。接种后检测到高水平的特异性抗体。虽然疫苗效力试验表明获得了一定程度的保护，但仍观察到高死亡率。攻毒后幸存鱼类头肾组织的 qPCR 分析显示，接种疫苗和未接种疫苗的鱼类细菌数量没有差异。濒死和患病鱼类的临床症状包括皮肤和眼睛周围出现白点、鳍磨损和嘴和鳍基部发红。尽管产生了特异性抗体，但对实验性攻毒的保护作用相对较弱。造成这种情况的一个原因可能是，产生的特异性抗体本身不足以提供对鲈形目的完全保护。对体外暴露于巴斯德氏菌的头肾白细胞进行共聚焦和扫描电子显微镜检查，表明了病原体和白细胞之间的相互作用。结果表明，除了体液抗体之外，免疫系统的某些部分可能对巴氏杆菌病的保护很重要。我们的综合结果强调了需要进一步研究巴斯德氏菌与鲈形目之间的宿主-病原体相互作用。
