Ellul Rebecca Marie, Kalatzis Panos G, Frantzen Cyril, Haugland Gyri Teien, Gulla Snorre, Colquhoun Duncan John, Middelboe Mathias, Wergeland Heidrun Inger, Rønneseth Anita
Department of Biological Sciences, University of Bergen, N-5006 Bergen, Norway.
Department of Biology, Marine Biological Section, University of Copenhagen, DK-3000 Helsingør, Denmark.
Microorganisms. 2021 Jun 4;9(6):1215. doi: 10.3390/microorganisms9061215.
Pasteurellosis in farmed lumpsuckers, , has emerged as a serious disease in Norwegian aquaculture in recent years. Genomic characterization of the causative agent is essential in understanding the biology of the bacteria involved and in devising an efficient preventive strategy. The genomes of two clinical isolates were sequenced (≈2.3 Mbp), and phylogenetic analysis confirmed their position as a novel species within the . In silico analyses revealed 11 genomic islands and 5 prophages, highlighting the potential of mobile elements as driving forces in the evolution of this species. The previously documented pathogenicity of is strongly supported by the current study, and 17 target genes were recognized as putative primary drivers of pathogenicity. The expression level of a predicted vaccine target, an uncharacterized adhesin protein, was significantly increased in both broth culture and following the exposure of to lumpsucker head kidney leucocytes. Based on in silico and functional analyses, the strongest gene target candidates will be prioritized in future vaccine development efforts to prevent future pasteurellosis outbreaks.
近年来，养殖圆鳍鱼的巴氏杆菌病已成为挪威水产养殖中的一种严重疾病。对病原体进行基因组特征分析对于了解相关细菌的生物学特性以及制定有效的预防策略至关重要。对两株临床分离株的基因组进行了测序（约2.3 Mbp），系统发育分析证实它们在[具体分类单元]内属于一个新物种。计算机分析揭示了11个基因组岛和5个原噬菌体，突出了移动元件作为该物种进化驱动力的潜力。当前研究有力地支持了先前记录的[病原体名称]的致病性，并且17个靶基因被认定为假定的主要致病驱动因素。一种预测的疫苗靶点，即一种未表征的粘附素蛋白，在肉汤培养以及[病原体名称]暴露于圆鳍鱼头肾白细胞后，其表达水平均显著升高。基于计算机分析和功能分析，在未来预防巴氏杆菌病爆发的疫苗开发工作中，将优先考虑最强的基因靶点候选物。
