Jiangxi Key Laboratory of Industrial Ceramics, College of Materials and Chemical Engineering, Pingxiang University, Pingxiang 337055, China.
Jiangxi Key Laboratory of Industrial Ceramics, College of Materials and Chemical Engineering, Pingxiang University, Pingxiang 337055, China.
Colloids Surf B Biointerfaces. 2020 Jan 1;185:110618. doi: 10.1016/j.colsurfb.2019.110618. Epub 2019 Nov 2.
Alkaline phosphatase (ALP) is an important biomarker for diagnosis, and the abnormal level of serum ALP is closely related to a variety of diseases. In present work, a ratiometric fluorescence probe based on hybrid nanoparticles CDs@YVO: Dy nanoparticle is introduced for alkaline phosphatase (ALP) activity determination. The CDs@YVO: Dy probe is constructed by the carbon dots (CDs) and YVO: Dy through a simple mixing method, in which the blue emission of CDs at 405 nm acts as the calibrated signal, the green emission of YVO: Dy at 574 nm decreased with the increasing targets ALP, and used as the output signal. In addition, the Cu and pyrophosphate (PPi) were also employed in this strategy to utilize the excellent fluorescnece quenching efficiency of Cu to the Dy ions emission of CDs@YVO: Dy, as well as the strong affinity of PPi for Cu. In the presence of analyts ALP, ALP catalyzes the hydrolysis of PPi, causing the release of Cu, resulting in the Dy ions emission quenched, while the CDs emission at 405 nm retained unchanged, based on this, we designed the off-on-off ratiometric fluorescence platform for ALP sensing. The experiment result shows that the ratio of F/F is linear to the concentration of ALP in arange of 0.05∼3000 U/L with a detection limit of 0.04 U/L, which is comparable or better than those reported fluorescence probe, especially the calibrated signal introduction of CDs can eliminate the background interference, improve the accuracy of proposed probe greatly. Furthermore, the discrimination of ALP enzyme inhibitor with the IC of 26 μM, and ALP concentration in real human serum sample has also demonstrated the applicability of CDs@YVO: Dy fluorescence sensor well.
碱性磷酸酶（ALP）是一种重要的诊断生物标志物，血清中 ALP 的异常水平与多种疾病密切相关。在目前的工作中，我们介绍了一种基于杂交纳米粒子 CDs@YVO：Dy 纳米粒子的比率荧光探针，用于碱性磷酸酶（ALP）活性的测定。该 CDs@YVO：Dy 探针是通过简单的混合方法由碳点（CDs）和 YVO：Dy 构建而成，其中 CDs 在 405nm 处的蓝色发射作为校准信号，YVO：Dy 在 574nm 处的绿色发射随着目标 ALP 的增加而减少，并作为输出信号。此外，本研究还利用 Cu 和焦磷酸盐（PPi）来增强 CDs@YVO：Dy 的荧光猝灭效率以及 PPi 与 Cu 的强亲和力。在分析物 ALP 的存在下，ALP 催化 PPi 的水解，导致 Cu 的释放，从而使 Dy 离子的发射猝灭，而 CDs 在 405nm 处的发射保持不变，基于此，我们设计了用于 ALP 传感的关-开-关比率荧光平台。实验结果表明，F/F 与 ALP 的浓度在 0.05∼3000 U/L 范围内呈线性关系，检测限为 0.04 U/L，与已报道的荧光探针相当或更好，特别是 CDs 的校准信号引入可以消除背景干扰，大大提高了探针的准确性。此外，用 IC 为 26μM 的 ALP 酶抑制剂进行区分，以及在真实的人血清样本中的 ALP 浓度也很好地证明了 CDs@YVO：Dy 荧光传感器的适用性。
