Friteau L, Jaffray P, Ronot X, Adolphe M
Laboratoire de Pharmacologie Cellulaire de l'Ecole Pratique des Hautes Etudes, Institut Biomédical des Cordeliers, Paris, France.
J Cell Physiol. 1988 Sep;136(3):514-8. doi: 10.1002/jcp.1041360317.
The cell-growth-inhibitory and phase-specific effects of D-penicillamine on cell-cycle progression were investigated using cell-proliferation patterns, quantitative cell-cycle analysis by flow cytometry, and determination of the mitotic index and binucleate cell fraction of normal (rabbit articular chondrocytes, L 809, rabbit fibroblasts) and transformed (HeLa, L 929) cells. D-penicillamine treatment resulted in an inhibition of growth within a dose range of 5 x 10(-4) M to 7.5 x 10(-3) M. Examination of DNA by flow cytometric analysis revealed that rabbit articular chondrocytes were preferentially arrested in the G0/1 phase of the cell cycle, whereas the other cell lines were blocked in the G2 + M phase; the increase in the proportion of cells with G2 + M DNA content was partially due to an enhancement of binucleate cells, resulting in a cytokinesis perturbation for HeLa and L 929 cells. These results showed that D-penicillamine affects cell proliferation through different events according to cell type.
利用细胞增殖模式、流式细胞术进行定量细胞周期分析以及测定正常细胞（兔关节软骨细胞、L 809、兔成纤维细胞）和转化细胞（HeLa、L 929）的有丝分裂指数和双核细胞比例，研究了D-青霉胺对细胞周期进程的细胞生长抑制和阶段特异性作用。D-青霉胺处理在5×10⁻⁴ M至7.5×10⁻³ M的剂量范围内导致生长抑制。通过流式细胞术分析DNA发现，兔关节软骨细胞优先停滞在细胞周期的G0/1期，而其他细胞系则停滞在G2 + M期；G2 + M期DNA含量细胞比例的增加部分归因于双核细胞的增多，导致HeLa和L 929细胞的胞质分裂受到干扰。这些结果表明，D-青霉胺根据细胞类型通过不同事件影响细胞增殖。
