D-青霉胺对培养的兔关节软骨细胞生长和细胞周期动力学的影响。
Effects of D-penicillamine on growth and cell cycle kinetics of cultured rabbit articular chondrocytes.
作者信息
Jaffray P, Ronot X, Adolphe M, Fontagne J, Lechat P
出版信息
Ann Rheum Dis. 1984 Apr;43(2):333-8. doi: 10.1136/ard.43.2.333.
The long-acting antirheumatic drug D-penicillamine was found to inhibit the growth of asynchronous cultures of rabbit articular chondrocytes. This inhibitory effect was dose-related between 5 X 10(-4) M and 5 X 10(-3) M and was time-dependent for a given dose. Flow cytometric analysis showed that drug exposure led to a slowdown in cell cycle progression. This was manifested as a decrease in the number of cells in S phase, due especially to an accumulation of cells in G0 G1 and also to a slight cessation of cell transit through G2 M. Recovery experiments showed that the effect is transitory and reversible. It is suggested that the articular chondrocyte is a target cell for D-penicillamine and that these cells have a D-penicillamine sensitive restriction point in the G0 G1 phase of the cell cycle and to a less extent in the G2 M phase.
长效抗风湿药物D-青霉胺被发现可抑制兔关节软骨细胞异步培养物的生长。在5×10⁻⁴ M至5×10⁻³ M之间，这种抑制作用与剂量相关，且对于给定剂量具有时间依赖性。流式细胞术分析表明，药物暴露导致细胞周期进程放缓。这表现为S期细胞数量减少，尤其是由于G0/G1期细胞积累以及细胞通过G2/M期略有停滞。恢复实验表明，这种作用是短暂且可逆的。有人提出，关节软骨细胞是D-青霉胺的靶细胞，并且这些细胞在细胞周期的G0/G1期有一个对D-青霉胺敏感的限制点，在G2/M期的程度较小。
Zotero 插件