College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Mar Pollut Bull. 2020 Jan;150:110601. doi: 10.1016/j.marpolbul.2019.110601. Epub 2019 Nov 6.
Endocrine-disrupting pollutants in marine environments have aroused great concern for their adverse effects on the reproduction of marine organisms. This study aimed to seek promising biomarkers for estrogenic/androgenic chemicals. First, two possible male-specific genes, SRY-box containing gene 9a2 (sox9a2) and gonadal soma-derived factor (gsdf), were cloned from marine medaka (Oryzias melastigma). Then the responses of sox9a2, gsdf, choriogenin (chgH and chgL), vitellogenin (vtg1 and vtg2), and cytochrome P450 aromatase (cyp19a and cyp19b) were investigated after exposure to 17α-ethynylestradiol (EE) and 17β-trenbolone (TB) at 2, 10, and 50 ng/L. The results showed that gsdf was specifically expressed in the testes and easily induced in the ovaries after TB exposure, indicating that gsdf was a potential biomarker of environmental androgens. ChgL was a useful biomarker of weak estrogen pollution for its high sensitivity to low levels of EE. In addition, both EE and TB exposure damaged gonadal structures and inhibited gonadal development.
海洋环境中的内分泌干扰污染物对海洋生物的繁殖产生了不利影响，引起了极大的关注。本研究旨在寻找有潜力的用于检测雌激素/雄激素类化学物质的生物标志物。首先，我们从海洋青鳉（Oryzias melastigma）中克隆了两个可能的雄性特异性基因，即包含 SRY 框的基因 9a2（sox9a2）和性腺体衍生因子（gsdf）。然后，我们研究了 sox9a2、gsdf、卵黄蛋白原（chgH 和 chgL）、卵黄蛋白（vtg1 和 vtg2）和细胞色素 P450 芳香化酶（cyp19a 和 cyp19b）在暴露于 17α-乙炔雌二醇（EE）和 17β-群勃龙（TB）2、10 和 50ng/L 后的反应。结果表明，gsdf 特异性表达于睾丸，并且在 TB 暴露后容易在卵巢中诱导表达，这表明 gsdf 是一种潜在的环境雄激素生物标志物。ChgL 是一种有用的弱雌激素污染生物标志物，因为它对 EE 的低浓度具有高灵敏度。此外，EE 和 TB 暴露均损害了性腺结构并抑制了性腺发育。
