Department of Municipal and Environmental Engineering, Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, Beijing Jiaotong University, Beijing, 100044, China; Laboratory of Quality and Safety Risk Assessments for Agro-products on Environmental Factors (Beijing), Ministry of Agriculture and Rural Affairs, 100029, China.
Laboratory of Quality and Safety Risk Assessments for Agro-products on Environmental Factors (Beijing), Ministry of Agriculture and Rural Affairs, 100029, China; Beijing Municipal Station of Agro-Environmental Monitoring, 100029, China.
Environ Pollut. 2020 Jan;256:113512. doi: 10.1016/j.envpol.2019.113512. Epub 2019 Oct 31.
Growing evidence shows plants are at risks of exposure to various per- and polyfluoroalkyl substances (PFASs), however the phytotoxicity induced by these compounds remains largely unknown on the molecular scale. Here, lettuce exposed to both perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) at different concentrations (500, 1000, 2000 and 5000 ng/L) in hydroponic media was investigated via metabolomics. Under the co-exposure conditions, the growth and biomass were not affected by PFOA and PFOS, but metabolic profiles of mineral elements and organic compounds in lettuce leaves were significantly altered. The contents of Na, Mg, Cu, Fe, Ca and Mo were decreased 1.8%-47.8%, but Zn was increased 7.4%-24.2%. The metabolisms of amino acids and peptides, fatty acids and lipids were down-regulated in a dose-dependent manner, while purine and purine nucleosides were up-regulated, exhibiting the stress response to PFOA and PFOS co-exposure. The reduced amounts of phytol (14.8%-77.0%) and abscisic acid (60.7%-73.8%) indicated the alterations in photosynthesis and signal transduction. The metabolism of (poly)phenol, involved in shikimate-phenylpropanoid pathway and flavonoid branch pathway, was strengthened, to cope with the stress of PFASs. As the final metabolites of (poly)phenol biosynthesis, the abundance of various antioxidants was changed. This study offers comprehensive insight of plant response to PFAS co-exposure and enhances the understanding in detoxifying mechanisms.
越来越多的证据表明，植物面临着接触各种全氟和多氟烷基物质（PFAS）的风险，但这些化合物在分子水平上对植物的毒性仍知之甚少。在这里，通过代谢组学研究了在水培介质中暴露于不同浓度（500、1000、2000 和 5000ng/L）全氟辛酸（PFOA）和全氟辛烷磺酸（PFOS）的生菜。在共暴露条件下，PFOA 和 PFOS 对生菜的生长和生物量没有影响，但生菜叶片中矿物质元素和有机化合物的代谢谱发生了显著改变。Na、Mg、Cu、Fe、Ca 和 Mo 的含量分别降低了 1.8%-47.8%，而 Zn 的含量增加了 7.4%-24.2%。氨基酸和肽、脂肪酸和脂质的代谢呈剂量依赖性下调，而嘌呤和嘌呤核苷则上调，表现出对 PFOA 和 PFOS 共暴露的应激反应。植醇（14.8%-77.0%）和脱落酸（60.7%-73.8%）的减少表明光合作用和信号转导的改变。（多）酚代谢途径，涉及莽草酸-苯丙氨酸途径和类黄酮分支途径，被加强，以应对 PFASs 的压力。作为（多）酚生物合成的最终代谢物，各种抗氧化剂的丰度发生了变化。本研究全面了解了植物对 PFAS 共暴露的反应，增强了对解毒机制的理解。
