Kitagawa N
Interaction Chemicals Inc., Mountain View, CA 94043.
J Chromatogr. 1988 Jun 29;443:133-41. doi: 10.1016/s0021-9673(00)94788-5.
A new, macroporous, synthetic polymer, Hydrophase HP-PEI, having highly hydrophilic surface characteristics enhanced by evenly distributed polyethyleneimine groups on its surface, was evaluated as an high-performance ion-exchange chromatographic packing material for the separation of proteins and nucleotides. A steel column (100 mm x 7.8 mm I.D.) packed with this material produces more than 33,000 plates per m. This base polymer included large proteins, such as thyroglobulin and apoferritin, in a size-exclusion mode. With various proteins it gave high recovery, high resolution and high capacity.
一种新型大孔合成聚合物——水相HP-PEI，其表面具有高度亲水性，表面均匀分布的聚乙烯亚胺基团增强了这种亲水性，该聚合物被评估为用于分离蛋白质和核苷酸的高性能离子交换色谱填充材料。填充这种材料的钢柱（内径100毫米×7.8毫米）每米产生超过33000个理论塔板数。这种基础聚合物在尺寸排阻模式下能容纳诸如甲状腺球蛋白和脱铁铁蛋白等大分子蛋白质。对于各种蛋白质，它都具有高回收率、高分辨率和高容量。
