Boschetti E, Guerrier L, Girot P, Horvath J
BioSepra SA, Villeneuve la Garenne, France.
J Chromatogr B Biomed Appl. 1995 Feb 3;664(1):225-31. doi: 10.1016/0378-4347(94)00406-u.
HyperD ion-exchange media combine the mechanical strength of a rigid polystyrene-mineral composite skeleton with the high protein-binding capacity of a three-dimensional soft gel located inside the skeleton. The skeleton solid matrix is completely filled with functionalized, highly hydrophilic, chemically stable ion-exchange hydrogels. These materials gave very efficient columns for protein separation with superior dynamic capacity, high resolving power and excellent protein recovery. Various protein mixtures were used to study the chromatographic performance of these new stationary phases. Comparisons between different particle size packing materials demonstrated the potential of this ion-exchange material for use on a large scale.
HyperD离子交换介质将刚性聚苯乙烯-矿物复合骨架的机械强度与位于骨架内部的三维软凝胶的高蛋白结合能力结合在一起。骨架固体基质完全填充有功能化、高度亲水、化学稳定的离子交换水凝胶。这些材料为蛋白质分离提供了非常高效的色谱柱，具有卓越的动态容量、高分辨率和出色的蛋白质回收率。使用了各种蛋白质混合物来研究这些新型固定相的色谱性能。不同粒径填充材料之间的比较证明了这种离子交换材料大规模应用的潜力。
