Plachot M, Veiga A, Montagut J, de Grouchy J, Calderon G, Lepretre S, Junca A M, Santalo J, Carles E, Mandelbaum J
U173 INSERM, Hôpital Necker, Paris, France.
Hum Reprod. 1988 Jul;3(5):627-35. doi: 10.1093/oxfordjournals.humrep.a136758.
A multicentric study was carried out to analyse in a large series: (i) the chromosomal status of unfertilized oocytes, (ii) errors at fertilization and (iii) the chromosomal complement of cleaved embryos. Parameters such as type of sterility, maternal age, stimulation treatment, doses of gonadotrophins administered and oocyte preincubation time before insemination were studied in relation to the incidence of chromosome abnormalities. Twenty-six per cent of the unfertilized oocytes and 29.2% of the embryos had chromosome anomalies. Maternal age significantly increased the rate of aneuploidy in oocytes: 38% in patients over 35 years (versus 24% in younger patients). Fertilization-related abnormalities were significant, i.e. 1.6% parthenogenesis and 6.4% polyploidy. Unexplained infertility was correlated with an increase in the rate of parthenogenesis (4.2%) when compared with tubal infertility (1.2%). Triploidy was found to be correlated with three parameters. A lower rate of triploidy was observed in the group of couples referred because of male sterility (1.9% versus 6.3% for tubal sterility), in HMG-treated patients (2.4% versus 7% with analogues of LHRH/HMG) and with a short 2-h preincubation time before insemination (3% versus 7.2% for greater than 2 h). A general model for natural selection against embryos carrying a chromosome imbalance was proposed.
开展了一项多中心研究，以对大量样本进行分析：(i) 未受精卵母细胞的染色体状态；(ii) 受精时的错误情况；(iii) 卵裂胚胎的染色体组成。研究了诸如不育类型、母亲年龄、刺激治疗、促性腺激素给药剂量以及授精前卵母细胞预孵育时间等参数与染色体异常发生率的关系。26%的未受精卵母细胞和29.2%的胚胎存在染色体异常。母亲年龄显著增加了卵母细胞非整倍体率：35岁以上患者中为38%（年轻患者中为24%）。与受精相关的异常情况显著，即孤雌生殖占1.6%，多倍体占6.4%。与输卵管性不育（1.2%）相比，不明原因的不育与孤雌生殖率增加（4.2%）相关。发现三倍体与三个参数相关。在因男性不育转诊的夫妇组中观察到较低的三倍体率（1.9%，输卵管性不育组为6.3%），在接受HMG治疗的患者中（2.4%，促性腺激素释放激素类似物/HMG治疗组为7%），以及在授精前2小时短时间预孵育的情况下（3%，预孵育时间大于2小时组为7.2%）。提出了一个针对携带染色体不平衡胚胎的自然选择通用模型。
