Fujian Engineering and Technology Research Center for Comprehensive Utilization of Marine Products Waste, Fuzhou University, Fuzhou, Fujian 350108, China; Fuzhou Industrial Technology Innovation Center for High Value Utilization of Marine Products, Fuzhou University, Fuzhou, Fujian 350108, China.
Fujian Engineering and Technology Research Center for Comprehensive Utilization of Marine Products Waste, Fuzhou University, Fuzhou, Fujian 350108, China; Fuzhou Industrial Technology Innovation Center for High Value Utilization of Marine Products, Fuzhou University, Fuzhou, Fujian 350108, China.
Comp Biochem Physiol B Biochem Mol Biol. 2020 Feb;240:110385. doi: 10.1016/j.cbpb.2019.110385. Epub 2019 Nov 7.
Lustrin A is the first nacre protein with specific structure and amino acid residue content that was identified in abalone; since its identification, homologs have been found in several abalone species. In this study, we isolated and cloned the complete cDNA of Lustrin A from Haliotis discus hannai, which was named Hdh-Lustrin A. Hdh-Lustrin A has characteristic cysteine- and proline-rich domains, glycine- and serine-rich domains, and a whey acidic protein (WAP)-like C-terminus. The cysteine- and proline-rich domains showed internal similarity repeats that arrayed in gene coding region, and the phylogenetic tree of these repeats indicated that the similarity of structural repetitive unit components in different abalone species, reflecting their evolutionary distance. A tissue distribution analysis showed that the mRNA level of Hdh-Lustrin A has tissue-specific expression in mantle. Under lipopolysaccharide (LPS) challenge, Hdh-Lustrin A showed a significantly increase, while it showed a more complex pattern with two peaks in the process of shell regeneration. Moreover, acidification and warming raised the expression level of Hdh-Lustrin A in shell regeneration in two different manners; acidification raised the gene expression in quick response, in contrast the long run in warming treatment. Similar pattern also has been detected in immune reaction and the thermal treatments. These results suggest that the Hdh-Lustrin A is a nacre protein, which can be distinguished by its cysteine- and proline-rich domain. It involves in shell regeneration and innate immunity in abalone, and its expression pattern during shell regeneration can be disrupted by physicochemical properties of the environment.
珍珠质蛋白 A 是首个在鲍鱼中被鉴定出具有特定结构和氨基酸残基含量的珍珠质蛋白；自其被鉴定以来，已在多个鲍鱼物种中发现了同源物。本研究从皱纹盘鲍中分离并克隆了珍珠质蛋白 A 的全长 cDNA，命名为 Hdh-Lustrin A。Hdh-Lustrin A 具有特征性的半胱氨酸和脯氨酸丰富域、甘氨酸和丝氨酸丰富域以及乳清酸性蛋白 (WAP)-样 C 末端。半胱氨酸和脯氨酸丰富域在基因编码区中显示出内部相似重复，这些重复的系统发育树表明不同鲍鱼物种结构重复单元成分的相似性，反映了它们的进化距离。组织分布分析表明，Hdh-Lustrin A 的 mRNA 水平在套膜中具有组织特异性表达。在脂多糖 (LPS) 刺激下，Hdh-Lustrin A 的表达水平显著增加，而在贝壳再生过程中，其表达模式更为复杂，呈现两个峰值。此外，酸化和升温以两种不同的方式提高了贝壳再生过程中 Hdh-Lustrin A 的表达水平；酸化以快速反应的方式提高基因表达，而升温处理则以长期反应的方式提高基因表达。在免疫反应和热处理中也检测到了类似的模式。这些结果表明，Hdh-Lustrin A 是一种珍珠质蛋白，可以通过其半胱氨酸和脯氨酸丰富域来区分。它参与了鲍鱼的贝壳再生和先天免疫，其在贝壳再生过程中的表达模式可以被环境的物理化学性质所破坏。
