Sharker Md Rajib, Kim Soo Cheol, Hossen Shaharior, Sumi Kanij Rukshana, Choi Sang Ki, Choi Kap Seong, Kho Kang Hee
Department of Fisheries Science, College of Fisheries and Ocean Sciences, Chonnam National University, Yeosu, South Korea.
Department of Fisheries Biology and Genetics, Faculty of Fisheries, Patuakhali Science and Technology University, Patuakhali, Bangladesh.
Front Mol Biosci. 2021 Apr 15;8:655115. doi: 10.3389/fmolb.2021.655115. eCollection 2021.
Carbonic anhydrases (CAs) are universal zinc ion containing metalloenzymes that play a pivotal role in various physiological processes. In this study, a CA I (designated as Hdh CA I) was isolated and characterized from the mantle tissue of Pacific abalone, . The full-length cDNA sequence of Hdh CA I was 1,417-bp in length, encoding a protein of 337 amino acids with molecular weight of 37.58 kDa. Hdh CA I sequence possessed a putative signal peptide of 22 amino acids and a CA catalytic function domain. The predicted protein shared 94 and 78% sequence identities with and CA I, respectively. Results of phylogenetic analysis indicated that Hdh CA I was evolutionarily close to CA I of and with high bootstrap values. Significantly higher levels of Hdh CA I mRNA transcript were found in mantle than other examined tissues. hybridization results showed strong hybridization signals in epithelial cells of the dorsal mantle pallial, an area known to synthesize and secrete proteins responsible for the nacreous layer formation of shell. This is the first study on Hdh CA I in and the results may contribute to further study its physiological functions in shell biomineralization of abalone.
碳酸酐酶（CAs）是普遍存在的含锌离子金属酶，在各种生理过程中起关键作用。在本研究中，从太平洋鲍鱼的外套膜组织中分离并鉴定了一种碳酸酐酶I（命名为Hdh CA I）。Hdh CA I的全长cDNA序列长度为1417bp，编码一个由337个氨基酸组成的蛋白质，分子量为37.58 kDa。Hdh CA I序列具有一个22个氨基酸的推定信号肽和一个CA催化功能域。预测的蛋白质与[具体物种1]和[具体物种2]的CA I分别具有94%和78%的序列同一性。系统发育分析结果表明，Hdh CA I在进化上与[具体物种1]和[具体物种2]的CA I关系密切，自展值较高。在外套膜中发现的Hdh CA I mRNA转录水平显著高于其他检测组织。原位杂交结果显示，在背侧外套膜鳃上皮细胞中有强烈的杂交信号，该区域已知可合成和分泌负责贝壳珍珠层形成的蛋白质。这是首次对[物种名称]中的Hdh CA I进行研究，其结果可能有助于进一步研究其在鲍鱼贝壳生物矿化中的生理功能。
