Fujian Engineering and Technology Research Center for Comprehensive Utilization of Marine Products Waste, Fuzhou University, Fujian, Fuzhou, 350108, China; Fuzhou Industrial Technology Innovation Center for High Value Utilization of Marine Products, Fuzhou University, Fujian, Fuzhou, 350108, China.
Fujian Engineering and Technology Research Center for Comprehensive Utilization of Marine Products Waste, Fuzhou University, Fujian, Fuzhou, 350108, China; Fuzhou Industrial Technology Innovation Center for High Value Utilization of Marine Products, Fuzhou University, Fujian, Fuzhou, 350108, China.
Mar Environ Res. 2020 Dec;162:105114. doi: 10.1016/j.marenvres.2020.105114. Epub 2020 Aug 28.
Ocean warming and acidification caused by global climate change interferes with the shell growth of mollusks. In abalone Haliotis discus hannai, the microstructural changes in the shell under stress are unclear, and the effect of thermal stress on biomineralization is unknown. The lack of gene information has also hampered the study of abalone biomineralization mechanisms. In this study, the microstructure of reconstructed shell in H. discus hannai was observed to determine the effects of thermal and acidification stress on shell growth. Three nacre protein genes, Hdh-AP7, Hdh-AP24, and Hdh-perlustrin, were characterized, and their expression pattern during shell repair was measured under thermal and acidification stress and compared with those of two known biomineralization-related genes, Hdh-AP-1 and Hdh-defensin. The stress resulted in aragonite plates with corroded or irregular microstructures. The gene expression of two nacre proteins (Hdh-AP7 and Hdh-AP24), which directly induce crystal formation, were more sensitive to thermal stress than to acidification, but the expression of the regulatory nacre protein (Hdh-perlustrin) and the two known genes (Hdh-AP-1 and Hdh-defensin), which are also related to immunity, showed an interlinked, complex pattern change. We concluded that high temperature and acidification damages the shell microstructure by disturbing the expression pattern of biomineralization-related genes.
全球气候变化导致的海洋升温与酸化会干扰贝类的壳生长。在盘鲍（Haliotis discus hannai）中，其壳在压力下的微观结构变化尚不清楚，热应激对生物矿化的影响也尚不清楚。基因信息的缺乏也阻碍了对鲍生物矿化机制的研究。在这项研究中，观察了重构壳的微观结构，以确定热和酸化应激对壳生长的影响。对三个珍珠层蛋白基因（Hdh-AP7、Hdh-AP24 和 Hdh-perlustrin）进行了表征，并测量了它们在热和酸化应激下修复壳时的表达模式，并与两个已知的生物矿化相关基因（Hdh-AP-1 和 Hdh-defensin）进行了比较。应激导致方解石板的微观结构被腐蚀或不规则。两个珍珠层蛋白基因（Hdh-AP7 和 Hdh-AP24）的基因表达对热应激比对酸化更敏感，而调节珍珠层蛋白（Hdh-perlustrin）和两个已知基因（Hdh-AP-1 和 Hdh-defensin）的基因表达则表现出相互关联、复杂的模式变化。我们得出结论，高温和酸化通过干扰生物矿化相关基因的表达模式来破坏壳的微观结构。
