Pindak F F, Mora de Pindak M, Gardner W A, Abee C R
Department of Pathology, University of South Alabama College of Medicine, Mobile 36617.
J Clin Microbiol. 1988 Aug;26(8):1460-3. doi: 10.1128/jcm.26.8.1460-1463.1988.
Tritrichomonas mobilensis is a recently described enteric protozoon of squirrel monkeys. An earlier report identified one of the metabolic products of this organism as a lectinlike hemagglutinin. Its further properties were determined in this study. Culture supernatants of T. mobilensis FP4190 were concentrated by ultrafiltration through a membrane with 100,000-molecular-weight cutoff. High titers of agglutinin against human erythrocytes were obtained. Incubation at 70 degrees C for 15 min resulted in complete inactivation. Exposure to 56 degrees C for 30 min was without effect, and only partial loss of activity was obtained during incubation for up to 18 h. Maintenance at pH 4 to 9 for 4 h at room temperature had no deleterious effect. Apparent degradation of the hemagglutinin was achieved by 18 h of contact with proteinase K, but trypsin and collagenase were essentially ineffective. Papain increased the sensitivity of the test. In the presence of this enzyme hemagglutinin was demonstrated also in cultures of Tritrichomonas foetus and Tritrichomonas augusta but not in those of Pentatrichomonas hominis or Trichomonas vaginalis.
活动三毛滴虫是最近报道的松鼠猴肠道原生动物。早期报告鉴定出该生物体的一种代谢产物为类凝集素血凝素。本研究确定了其进一步的特性。活动三毛滴虫FP4190的培养上清液通过截留分子量为100,000的膜进行超滤浓缩。获得了针对人红细胞的高滴度凝集素。在70℃孵育15分钟导致完全失活。暴露于56℃30分钟无影响，在长达18小时的孵育过程中仅出现部分活性丧失。在室温下于pH 4至9维持4小时没有有害影响。与蛋白酶K接触18小时可使血凝素明显降解，但胰蛋白酶和胶原酶基本无效。木瓜蛋白酶增加了试验的敏感性。在这种酶存在的情况下，胎儿三毛滴虫和奥古斯塔三毛滴虫的培养物中也显示出血凝素，但人五毛滴虫或阴道毛滴虫的培养物中未显示。
