使用无血清培养基在悬浮培养中生产慢病毒载体用于CAR-T细胞的转导
Lentiviral Vector Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells.
作者信息
Do Minh Aline, Tran Michelle Yen, Kamen Amine A
机构信息
Department of Bioengineering, McGill University, Montreal, QC, Canada.
出版信息
Methods Mol Biol. 2020;2086:77-83. doi: 10.1007/978-1-0716-0146-4_6.
PMID:31707669
Abstract
The production of lentiviral vectors (LVs) in human embryonic kidney 293 (HEK293) cells using serum-free medium in a suspension culture for the transduction of chimeric antigen receptor T-cells (CAR-T) can be achieved by different methods. This chapter describes LV production by transient transfection, induction of stable packaging cell lines, and induction of stable producer cell lines.
摘要
利用无血清培养基在悬浮培养的人胚肾293（HEK293）细胞中生产慢病毒载体（LVs）用于嵌合抗原受体T细胞（CAR-T）的转导可通过不同方法实现。本章介绍了通过瞬时转染、诱导稳定包装细胞系和诱导稳定生产细胞系来生产LVs的方法。
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