Moço Pablo Diego, de Abreu Neto Mário Soares, Fantacini Daianne Maciely Carvalho, Picanço-Castro Virgínia
Center for Cell-Based Therapy CTC, Regional Blood Center of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
Methods Mol Biol. 2020;2086:69-76. doi: 10.1007/978-1-0716-0146-4_5.
Advances in the use of lentiviral vectors for gene therapy applications have created a need for large-scale manufacture of clinical-grade viral vectors for transfer of genetic materials. Lentiviral vectors can transduce a wide range of cell types and integrate into the host genome of dividing and nondividing cells, resulting in long-term expression of the transgene both in vitro and in vivo. In this chapter, we present a method to transfect human cells, creating an easy platform to produce lentiviral vectors for CAR-T cell application.
用于基因治疗的慢病毒载体使用方面的进展，使得大规模生产用于遗传物质转移的临床级病毒载体成为必要。慢病毒载体可以转导多种细胞类型，并整合到分裂细胞和非分裂细胞的宿主基因组中，从而在体外和体内实现转基因的长期表达。在本章中，我们介绍一种转染人类细胞的方法，创建一个易于操作的平台来生产用于嵌合抗原受体T细胞（CAR-T细胞）应用的慢病毒载体。
