Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
Adv Exp Med Biol. 2019;1163:65-87. doi: 10.1007/978-981-13-8719-7_4.
An allosteric mechanism refers to the biological regulation process wherein macromolecules propagate the effect of ligand binding at one site to a spatially distant orthosteric locus, thus affecting activity. The theory has remained a trending topic in biology research for over 50 years, since the understanding of allostery is fundamental for gleaning numerous biological processes and developing new drug therapies. In the past two decades, the allosteric paradigm has evolved into more descriptive models, with ever-expanding amounts of experimental data pertaining to newly identified allosteric molecules. The AlloSteric Database (ASD, accessible at http://mdl.shsmu.edu.cn/ASD ), which is a comprehensive knowledge repository, has provided the public with integrated information encompassing allosteric proteins, modulators, sites, pathways, and networks to investigate allostery since 2009. In this chapter, we introduce the history and usage of the ASD and give attention to specific applications that have benefited from the ASD.
变构机制是指生物调节过程中，大分子将配体结合在一个位置的效应传递到空间上相隔的正位点，从而影响活性。自 50 多年前该理论提出以来，它一直是生物学研究的热门话题，因为对变构的理解是了解许多生物过程和开发新的药物治疗方法的基础。在过去的二十年中，变构范例已经发展成更具描述性的模型，与新确定的变构分子相关的实验数据不断增加。AlloSteric Database (ASD，可在 http://mdl.shsmu.edu.cn/ASD 访问) 自 2009 年以来一直是一个综合性知识库，为公众提供了包含变构蛋白、调节剂、位点、途径和网络的综合信息，以研究变构。在本章中，我们介绍了 ASD 的历史和用途，并关注了受益于 ASD 的特定应用。
