Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China; Risk Assessment Lab for Agro-products (Beijing), Ministry of Agriculture. PR China, Beijing, 100097, China; Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, Beijing, 100097, China.
Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China; Risk Assessment Lab for Agro-products (Beijing), Ministry of Agriculture. PR China, Beijing, 100097, China; Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, Beijing, 100097, China.
Anal Chim Acta. 2019 Dec 27;1092:75-84. doi: 10.1016/j.aca.2019.09.039. Epub 2019 Sep 16.
A novel microfluidic colorimetric immunoassay was developed using gold nanoparticles (GNPs) for indicating different concentrations of altenariol monomethyl ether (AME), and UV spectroscopy and smart phone imaging for monitoring color change of the GNPs. Norland Optical Adhesive 81 (NOA 81) was used for simple and rapid fabrication of the microfluidic chip. AME-BSA modified magnetic nanoparticles (MNPs-BSA-AME) were used as capture probe and the self-magnetism for rapid separation and purification. AME monoclonal antibodies modified gold nanoparticles (GNP-mAbs) which dried on conjugate pad were used as detection probe and the self-catalyst for signal amplification. Under the optimal conditions, the proposed microfluidic colorimetric immunoassay was able to detect AME as low as 12.5 pg/mL for UV spectroscopy (574 nm), and 200 pg/mL for smart phone imaging. The total analysis time is less than 15 min. The immunoassay also has a lower cross-reactivity to AME analogues. It was also evaluated by analyzing fruit samples spiked with AME. The recoveries ranged from 91.19% to 94.15% for UV spectroscopy, and from 90.63% to 93.9% for smart phone imaging. This method can be used for rapid, sensitive, low-cost and portable point-of care testing (POCT) of other mycotoxins or haptens in food samples.
一种新型的微流控比色免疫分析方法利用金纳米粒子（GNPs）来指示不同浓度的交替醇单甲醚（AME），并用紫外光谱和智能手机成像来监测 GNPs 的颜色变化。诺兰德光学胶 81（NOA 81）用于简单快速地制造微流控芯片。AME-BSA 修饰的磁性纳米粒子（MNPs-BSA-AME）被用作捕获探针，并利用其自磁性进行快速分离和纯化。AME 单克隆抗体修饰的金纳米粒子（GNP-mAbs）干燥在结合垫上作为检测探针，并利用其自催化剂进行信号放大。在最佳条件下，所提出的微流控比色免疫分析方法能够在紫外光谱（574nm）下检测到低至 12.5pg/mL 的 AME，在智能手机成像下检测到低至 200pg/mL 的 AME。总分析时间少于 15 分钟。该免疫分析方法对 AME 类似物的交叉反应性也较低。该方法还通过分析添加 AME 的水果样品进行了评估。紫外光谱的回收率范围为 91.19%至 94.15%，智能手机成像的回收率范围为 90.63%至 93.9%。该方法可用于快速、灵敏、低成本和便携式现场即时检测（POCT）食品样品中的其他真菌毒素或半抗原。
