Fay Cormac D, Wu Liang, Perez de Vargas Sansalvador Isabel M
School of Medical, Indigenous and Health Sciences, Faculty of Science, Medicine and Health, University of Wollongong, Wollongong, NSW 2522, Australia.
Melbourne Centre for Nanofabrication (MCN), Department of Materials Science and Engineering, Monash University, 22 Alliance Lane, Clayton, VIC 3168, Australia.
Sensors (Basel). 2025 Jun 20;25(13):3850. doi: 10.3390/s25133850.
This study provides a detailed comparison of three optical sensing approaches for colourimetric bio/chemical detection, focusing on cost, scalability, and performance. We examine laboratory-grade spectrophotometry, portable camera-based imaging, and low-cost LED photometry using Paired Emitter-Detector Diode (PEDD) charge-discharge methodology. Our findings reveal that while the LED-based PEDD system outperforms the other two methods in key sensory metrics-such as sensitivity, resolution, and limit of detection-its cost-effectiveness and scalability make it a promising solution for widespread industrial and field applications. Compared to the spectrophotometer, the LED/PEDD approach demonstrates improvements in measurement range (×16.39), dynamic range (×147.06), accuracy (×1.79), and sensitivity (×107.53). The results highlight the potential for industrial-scale adoption of LED photometry, especially for cost-effective applications in bio/chemical sensing sectors.
本研究对用于比色生物/化学检测的三种光学传感方法进行了详细比较，重点关注成本、可扩展性和性能。我们使用配对发射极-探测器二极管（PEDD）充放电方法研究了实验室级分光光度法、基于便携式相机的成像法以及低成本LED光度法。我们的研究结果表明，虽然基于LED的PEDD系统在关键传感指标（如灵敏度、分辨率和检测限）方面优于其他两种方法，但其成本效益和可扩展性使其成为广泛的工业和现场应用的一个有前景的解决方案。与分光光度计相比，LED/PEDD方法在测量范围（×16.39）、动态范围（×147.06）、准确度（×1.79）和灵敏度（×107.53）方面都有改进。结果突出了LED光度法在工业规模应用方面的潜力，特别是在生物/化学传感领域具有成本效益的应用。
