Mild K H, Løvtrup S, Forslind E
J Exp Biol. 1979 Dec;83:305-14. doi: 10.1242/jeb.83.1.305.
The exchange of isotopic water, 2H2O and H2(18)O has been studied in amphibian eggs. The experiments were carried out with ovarian and body cavity eggs of Rana temporaria and unfertilized eggs of Ambystoma mexicanum. The cytoplasmic diffusion coefficient for H2(18)O was found to be 4.6 x 10(-6) cm2/s, somewhat higher than that for 2H2O, 3.4 x 10(-6) cm2/s. The total change in reduced weight, delta RW, during the isotope experiments was compared with the total amount of water in the egg cell, m. The ratio delta RW/m was significantly higher than would be expected from calculations using ordinary water density values. The results are discussed in terms of different phases of structured cell water.
已对两栖类卵中同位素水（2H2O和H2(18)O）的交换进行了研究。实验是用林蛙的卵巢卵和体腔卵以及美西钝口螈的未受精卵进行的。发现H2(18)O在细胞质中的扩散系数为4.6×10(-6) cm2/s，略高于2H2O的扩散系数3.4×10(-6) cm2/s。在同位素实验期间，将减重的总变化量δRW与卵细胞中的总水量m进行了比较。δRW/m的比值明显高于使用普通水密度值计算所预期的值。根据结构化细胞水的不同阶段对结果进行了讨论。
