Ganjiani Vahid, Ahmadi Nasrollah, Raayat Jahromi Alireza
School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Veterinary Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Andrologia. 2020 Mar;52(2):e13469. doi: 10.1111/and.13469. Epub 2019 Nov 11.
This project aimed to examine Stevia rebaudiana aqueous extract protective effects on testicular ischaemia/reperfusion injury of rats. Forty rats were randomly divided into five groups: (1) sham group, (2) torsion/detorsion group, (3 and 4) low and high doses treatment groups received S. rebaudiana extract intraperitoneally 30 min before detorsion by 500 and 1,000 mg/kg respectively, and (5) healthy group received the extract by 1,000 mg/kg. In this study, left testes were rotated 2 hr, reperfusion period took long 5 hr, and then orchiectomy was performed. Histopathological and biochemical evaluations of testicular tissue samples were performed. Histopathologically, sham and healthy groups exhibited normal seminiferous tubules. Germinal cell necrosis, interstitial oedema, haemorrhage and congestion were seen in torsion/detorsion group. Testicular tissues of both treatment groups revealed lower histopathological alterations. Significant higher malondialdehyde level was observed in torsion/detorsion group than sham and healthy groups (p < .05). Compared with torsion/detorsion group, S. rebaudiana extract significantly reduced malondialdehyde level in treatment groups (p < .05). Torsion/detorsion group had significantly lower glutathione peroxidase and superoxide dismutase activities than sham and healthy groups, and these parameters showed significant increase in treatment groups compared with torsion/detorsion group (p < .05). The results revealed S. rebaudiana has this potential to protect the testes from ischaemia/reperfusion injury.
本项目旨在研究甜叶菊水提取物对大鼠睾丸缺血/再灌注损伤的保护作用。40只大鼠随机分为五组：(1)假手术组；(2)扭转/去扭转组；(3)和(4)低剂量和高剂量治疗组，分别在去扭转前30分钟腹腔注射500和1000mg/kg的甜叶菊提取物；(5)健康组腹腔注射1000mg/kg的提取物。本研究中，左侧睾丸扭转2小时，再灌注5小时，然后进行睾丸切除术。对睾丸组织样本进行组织病理学和生化评估。组织病理学上，假手术组和健康组的生精小管正常。扭转/去扭转组可见生殖细胞坏死、间质水肿、出血和充血。两个治疗组的睾丸组织病理改变较轻。扭转/去扭转组的丙二醛水平显著高于假手术组和健康组(p<0.05)。与扭转/去扭转组相比，甜叶菊提取物显著降低了治疗组的丙二醛水平(p<0.05)。扭转/去扭转组的谷胱甘肽过氧化物酶和超氧化物歧化酶活性显著低于假手术组和健康组，与扭转/去扭转组相比治疗组的这些参数显著升高(p<0.05)。结果表明，甜叶菊具有保护睾丸免受缺血/再灌注损伤的潜力。
