Bahna S L, Strimas J H, Reed M A, Butcher B T
Department of Pediatrics, Louisiana State University School of Medicine, New Orleans.
J Allergy Clin Immunol. 1988 Sep;82(3 Pt 1):419-24. doi: 10.1016/0091-6749(88)90014-0.
We studied 19 young children (aged 15 to 39 months) who had had a systemic allergic reaction after imported fire ant (IFA) sting. Skin testing was performed with whole body extract (WBE) of both Solenopsis species; positive reactions to S. invicta was noted in 94% and to S. richteri in 100%. Serum was tested for total IgE level and for specific IgE antibodies by RAST with WBE and venom (V) of S. invicta. In vitro results were compared with those of 19 age and sex-matched atopic control subjects. The range of total serum IgE level was 31 to 1720 IU/ml (geometric mean 160 IU/ml), compared with 7.5 to 6756 IU/ml (geometric mean 85 IU/ml) in the control subjects. The difference between the two groups, however, was not statistically significant (p greater than 0.05). In the IFA-allergic group, the IFA WBE RAST mean binding was 13.5% (range 2.0% to 30.2%), and the IFA V RAST mean was 29.2% (range 7.8% to 46.7%). The control sera demonstrated low levels of activity to both IFA WBE RAST (0.7% to 4.8%) and IFA V RAST (0.7% to 5.9%). IFA WBE RAST demonstrated that 26% of the IFA-allergic patients had counts within the range of the atopic control subjects. In contrast, the IFA V RAST clearly separated all IFA-allergic patients from their age and sex-matched atopic control subjects. A scoring system for IFA V RAST is proposed. These results demonstrate superiority of IFA V over IFA WBE RAST in the diagnosis of allergy to S. invicta.
我们研究了19名（年龄在15至39个月之间）在被进口火蚁（IFA）蜇伤后出现全身过敏反应的幼儿。用两种火蚁属物种的全身提取物（WBE）进行皮肤试验；对入侵红火蚁的阳性反应率为94%，对黑火蚁的阳性反应率为100%。检测血清中的总IgE水平以及用入侵红火蚁的WBE和毒液（V）通过放射变应原吸附试验（RAST）检测特异性IgE抗体。将体外试验结果与19名年龄和性别匹配的特应性对照受试者的结果进行比较。血清总IgE水平范围为31至1720 IU/ml（几何平均值为160 IU/ml），而对照受试者为7.5至6756 IU/ml（几何平均值为85 IU/ml）。然而，两组之间的差异无统计学意义（p大于0.05）。在IFA过敏组中，IFA WBE RAST平均结合率为13.5%（范围为2.0%至30.2%），IFA V RAST平均结合率为29.2%（范围为7.8%至46.7%）。对照血清对IFA WBE RAST（0.7%至4.8%）和IFA V RAST（0.7%至5.9%）均显示出低水平活性。IFA WBE RAST显示，26% 的IFA过敏患者的计数在特应性对照受试者范围内。相比之下，IFA V RAST能清楚地将所有IFA过敏患者与其年龄和性别匹配的特应性对照受试者区分开来。提出了一种IFA V RAST评分系统。这些结果表明IFA V在诊断入侵红火蚁过敏方面优于IFA WBE RAST。
