Paull B R, Coghlan T H, Vinson S B
J Allergy Clin Immunol. 1983 May;71(5):448-53. doi: 10.1016/0091-6749(83)90460-8.
Levels of IgE antibody to fire ant whole body extract (FA-WBE) and fire ant venom (FAV) were evaluated in 60 patients with clinical histories of fire ant sting hypersensitivity and 11 frequently stung individuals without significant clinical reactivity to fire ant. An overlap of levels of IgE antibody to FA-WBE and FAV was seen between groups of patients with increasingly severe clinical reactivity; however, the group mean IgE antibody level of patients without significant clinical reactivity differed markedly from the group means of patients with more severe clinical reactivity (p less than 0.001). In 31 patients a good correlation (r = 0.6935) was found comparing FA-WBE IgE antibody level with FA-WBE skin tests, and in 26 patients a good correlation (r = 0.8564) was obtained comparing FAV IgE antibody levels with FAV skin tests. An excellent Spearman rank correlation coefficient (r = 0.9197) was found on comparison of FAV and FA-WBE IgE antibody levels in 71 individuals. Cross-reactivity of FAV and FA-WBE was demonstrated by the ability of these reagents to competitively inhibit the binding of IgE antibody to solid-phase FA-WBE and FAV in the radioallergosorbent test (RAST). From these results we conclude that FAV and FA-WBE both contain relevant allergens important in fire ant-allergic individuals and that skin tests and RAST are both valid as diagnostic tests for fire ant allergy. Finally, there is a good correlation between RAST and skin tests using FA-WBE or FAV as test reagents, since these reagents are highly cross-reactive as demonstrated by their ability to inhibit each other in the RAST.
在60例有火蚁叮咬过敏临床病史的患者以及11例经常被火蚁叮咬但对火蚁无明显临床反应的个体中，评估了针对火蚁全身提取物（FA-WBE）和火蚁毒液（FAV）的IgE抗体水平。在临床反应性逐渐加重的患者组之间，观察到针对FA-WBE和FAV的IgE抗体水平存在重叠；然而，无明显临床反应的患者组平均IgE抗体水平与临床反应性更严重的患者组平均水平有显著差异（p小于0.001）。在31例患者中，比较FA-WBE IgE抗体水平与FA-WBE皮肤试验发现有良好相关性（r = 0.6935），在26例患者中，比较FAV IgE抗体水平与FAV皮肤试验获得良好相关性（r = 0.8564）。在71名个体中比较FAV和FA-WBE IgE抗体水平时发现了极佳的Spearman等级相关系数（r = 0.9197）。在放射变应原吸附试验（RAST）中，这些试剂能够竞争性抑制IgE抗体与固相FA-WBE和FAV的结合，从而证明了FAV和FA-WBE的交叉反应性。根据这些结果我们得出结论，FAV和FA-WBE都含有对火蚁过敏个体重要的相关变应原，并且皮肤试验和RAST作为火蚁过敏的诊断试验都是有效的。最后，使用FA-WBE或FAV作为试验试剂时，RAST与皮肤试验之间有良好相关性，因为这些试剂在RAST中能够相互抑制，证明它们具有高度交叉反应性。
