Boxer M B, Bush R K, Harris K E, Patterson R, Pruzansky J J, Yang W H
Department of Medicine, Northwestern University Medical School, Chicago, Ill.
J Allergy Clin Immunol. 1988 Oct;82(4):622-6. doi: 10.1016/0091-6749(88)90974-8.
An immediate-type hypersensitivity reaction has been proposed as one possible mechanism in which metabisulfites (MBSs) cause reactions. As demonstrated with certain occupational chemicals, we proposed that MBS might conjugate with human proteins, such as human serum albumin, and then cause an immunologic response. Because we had identified no reactions to MBS at the Northwestern Allergy Service, we used sera from four patients reported elsewhere as having positive skin tests and positive oral challenges to sulfites. We attempted to demonstrate, both in vitro by ELISA and in vivo by passive cutaneous transfer to monkey, evidence for IgE-mediated hypersensitivity to MBSs. Our results demonstrated that there is evidence of IgE antibody by passive transfer for one patient studied, but no evidence of IgE antibody by ELISA to an MBS-albumin conjugate in any of the four patients. This study illustrates the complexities involved in the evaluation and mechanism of MBS-induced disease and the caution with which results must be interpreted.
速发型超敏反应被认为是偏重亚硫酸盐（MBSs）引发反应的一种可能机制。正如某些职业性化学物质所表明的那样，我们推测MBS可能与人的蛋白质（如人血清白蛋白）结合，进而引发免疫反应。由于我们在西北过敏服务中心未发现对MBS的反应，我们使用了其他地方报道的4名患者的血清，这些患者的皮肤试验和口服亚硫酸盐激发试验呈阳性。我们试图通过ELISA体外检测以及通过被动皮肤转移至猴子体内检测，来证明IgE介导的对MBSs超敏反应的证据。我们的结果表明，在研究的1名患者中通过被动转移有IgE抗体的证据，但在4名患者中的任何一人中，通过ELISA均未发现针对MBS -白蛋白结合物的IgE抗体证据。这项研究说明了评估MBS诱发疾病及其机制所涉及的复杂性，以及对结果进行解释时必须持有的谨慎态度。
