Sainte-Laudy J, Vallon C, Guérin J C
Département de pneumologie, Hôpital de la Croix-Rousse, Lyon.
Allerg Immunol (Paris). 1994 Apr;26(4):132-4, 137-8.
On the basis of the selection of a population of patients intolerant to sulfites by the clinical history, a simple blind oral provocation test and a basophil activation test, we explored the basophil activation reaction induced by sulfites after passive sensitisation of blood donors basophils. We demonstrated that the percentages of activation obtained with a non covalent reagent (MBS-HSA), a covalent reagent (sulfonyl-HSA) and the optimal concentration of an anti-IgE were not significantly different. Human basophil activation was negativated by heating the transferred sera and by competition with a monoclonal human IgE. We also observed mediator release (histamine and LTC4) with a low frequency, histamine release being strictly related to the carrier protein concentration. In two cases, sulfite specific IgE were detected by ELISA. These results are in favour of the specificity and the IgE dependent nature of basophil activation induced by sulfites.
基于通过临床病史、简单的盲法口服激发试验和嗜碱性粒细胞活化试验选择出对亚硫酸盐不耐受的患者群体，我们研究了在献血者嗜碱性粒细胞被动致敏后亚硫酸盐诱导的嗜碱性粒细胞活化反应。我们证明，使用非共价试剂（MBS-HSA）、共价试剂（磺酰-HSA）和抗IgE最佳浓度获得的活化百分比没有显著差异。通过加热转移的血清以及与单克隆人IgE竞争可使人类嗜碱性粒细胞活化受到抑制。我们还观察到介质释放（组胺和白三烯C4）的频率较低，组胺释放与载体蛋白浓度密切相关。在两例病例中，通过ELISA检测到了亚硫酸盐特异性IgE。这些结果支持亚硫酸盐诱导的嗜碱性粒细胞活化的特异性和IgE依赖性。
