McBride P, Bradley D, Kaliner M
Allergic Diseases Section, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md.
J Allergy Clin Immunol. 1988 Oct;82(4):638-46. doi: 10.1016/0091-6749(88)90977-3.
A new radioimmunoassay for the measurement of histamine in biologic fluids was evaluated. Assay selectivity and specificity were achieved by "succinyl-glycinamide derivatization" of histamine in samples to mimic the immunogen used to generate the monoclonal antibody. The assay exhibits a linear response from 0.1 to 5.0 ng/ml of histamine and the monoclonal antibody used has partial recognition of only N-methylhistamine (other than histamine). With minimal modifications, the assay can accurately measure histamine in plasma, urine, and buffer. Normal ranges for human subjects were established: plasma levels are 0.193 +/- 0.08 ng/ml (n = 40) and urine levels are 20.9 +/- 11.2 micrograms histamine/gm creatinine (n = 10).
对一种用于测量生物体液中组胺的新型放射免疫分析方法进行了评估。通过对样品中的组胺进行“琥珀酰甘氨酰胺衍生化”，以模拟用于生成单克隆抗体的免疫原，从而实现了分析的选择性和特异性。该分析方法在组胺浓度为0.1至5.0 ng/ml时呈现线性响应，所使用的单克隆抗体仅对N-甲基组胺（除组胺外）有部分识别能力。只需进行最少的修改，该分析方法就能准确测量血浆、尿液和缓冲液中的组胺。确定了人类受试者的正常范围：血浆水平为0.193±0.08 ng/ml（n = 40），尿液水平为20.9±11.2微克组胺/克肌酐（n = 10）。
