Houdi A A, Crooks P A, Van Loon G R, Schubert C A
College of Pharmacy, Division of Medicinal Chemistry and Pharmacognosy, University of Kentucky, Lexington 40536-0053.
J Pharm Sci. 1987 May;76(5):398-401. doi: 10.1002/jps.2600760512.
The determination of picomolar levels of histamine and its major metabolite, N tau-methylhistamine, in biological fluids was achieved using reversed-phase liquid chromatography coupled with electrochemical detection. A simple sample purification procedure for blood and urine samples was carried out prior to analysis using an Amberlite CG-50 cation-exchange resin, which afforded an excellent recovery of both compounds.
采用反相液相色谱结合电化学检测法测定生物体液中皮摩尔水平的组胺及其主要代谢物N-τ-甲基组胺。在分析之前，使用Amberlite CG-50阳离子交换树脂对血液和尿液样本进行了简单的样品纯化程序，该程序使两种化合物都能获得出色的回收率。
