Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 110029, India.
Department of Medical Oncology, IRCH, All India Institute of Medical Sciences, New Delhi, 110029, India.
Reprod Biol. 2019 Dec;19(4):322-328. doi: 10.1016/j.repbio.2019.11.002. Epub 2019 Nov 8.
With advances in therapeutic methods, there is a high survival rate among leukemia patients, of an extent more than 80%. However, chemotherapeutic drugs used to treat these patients have adverse effects on their overall health profile including fertility. The primary aim of this study was to identify differentially expressed proteins in seminal plasma of acute lymphoblastic leukemia (ALL) survivors compared to age-matched healthy controls, which can provide molecular basis of idiopathic infertility in such survivors. Differential proteome profiling was performed by 2D-differential in-gel electrophoresis, protein spots were identified by mass spectrometry and selective differentially expressed proteins (DEPs) were validated by western blotting and ELISA method. Out of eight DEPs identified, five proteins (isocitrate dehydrogenase 1, semenogelin 1, lactoferrin, prolactin-inducible protein, and human serum albumin) were upregulated and three (pepsinogen, prostate specific antigen and prostatic acid phosphatase) were downregulated. Expression profiles of these proteins are suggestive of reduction in semen quality in ALL survivors and can further be explored to determine their fertility status.
随着治疗方法的进步，白血病患者的存活率很高，超过 80%。然而，用于治疗这些患者的化疗药物会对他们的整体健康状况产生不良影响，包括生育能力。本研究的主要目的是鉴定与年龄匹配的健康对照组相比，急性淋巴细胞白血病 (ALL) 幸存者的精浆中差异表达的蛋白质，这些蛋白质可以为这些幸存者的特发性不育提供分子基础。通过 2D 差异凝胶电泳进行差异蛋白质组学分析，通过质谱鉴定蛋白质斑点，通过 Western blot 和 ELISA 方法验证选择性差异表达蛋白 (DEP)。在鉴定的八个 DEP 中，有五个蛋白（异柠檬酸脱氢酶 1、精液蛋白 1、乳铁蛋白、催乳素诱导蛋白和人血清白蛋白）上调，三个蛋白（胃蛋白酶原、前列腺特异性抗原和前列腺酸性磷酸酶）下调。这些蛋白质的表达谱提示 ALL 幸存者的精液质量下降，进一步研究这些蛋白质可以确定他们的生育能力状况。
