Li Shize, Zhang Meihua, Xu Ning, Lv Jingcai, Jiang Jianping, Liu Jing, Wei Gang, Wang Bin
Department of Food Science and Engineering, Maotai University, Renhuai 564500, China..
Zootaxa. 2019 Jul 2;4624(4):zootaxa.4624.4.7. doi: 10.11646/zootaxa.4624.4.7.
A new species of the microhylid frog genus Microhyla is described from the Fanjing Mountain of Guizhou Province, China. Phylogenetic analyses based on DNA sequences of the the mitochondrial 12S rRNA, 16S rRNA and COI genes supported the new species as an independent lineage, closely related to M. beilunensis, M. mixtura and M. okinavensis. The uncorrected genetic distance on 16S rRNA gene between the new species and its closest congeners M. beilunensis, M. mixtura and M. okinavensis are 3.5%, 4.6% and 4.6% respectively. The new species is distinguished from its congeners by a combination of the following morphological characters: (1) body of medium size (SVL 19.0-22.7 mm in males and 22.5-23.0 mm in females); (2) disk and dorsal median longitudinal groove on finger tips absent; (3) toe with rudimentary webbing at base; (4) disk with dorsal median longitudinal groove present at toe tips except for the toe I; (5) two metatarsal tubercles on palm; (6) tibiotarsal articulation reaching the level between eye to nostril when leg stretched forward; (7) a distinct V-shaped white stripe on the upper midsection. The new species is known only from the type locality, and thus the finding of it has contributed to the endemic species diversity of China. It is needed to take strategy to protect the species and habitats due to the increased threats of environmental changes.
一种新的姬蛙属小姬蛙被描述于中国贵州省梵净山。基于线粒体12S rRNA、16S rRNA和COI基因的DNA序列进行的系统发育分析支持该新物种为一个独立谱系，与北仑姬蛙、合征姬蛙和冲绳姬蛙亲缘关系密切。新物种与其最接近的同属物种北仑姬蛙、合征姬蛙和冲绳姬蛙在16S rRNA基因上的未校正遗传距离分别为3.5%、4.6%和4.6%。新物种与其同属物种的区别在于以下形态特征的组合：（1）中等体型（雄性体长19.0 - 22.7毫米，雌性体长22.5 - 23.0毫米）；（2）指尖无吸盘和背中纵沟；（3）趾基部有不发达的蹼；（4）除第一趾外，趾尖有背中纵沟的吸盘；（5）掌部有两个跖突；（6）腿向前伸展时，胫跗关节达到眼至鼻孔之间的水平；（7）上体中部有一条明显的V形白色条纹。该新物种仅知分布于模式产地，因此它的发现增加了中国特有物种的多样性。由于环境变化带来的威胁增加，需要采取策略保护该物种及其栖息地。
