Chen Ruonan, Chu Qingpo, Shen Chunyan, Tong Xian, Gao Siyuan, Liu Xinpeng, Zhou Bo, Schinckel Allan P
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907-2054, USA.
Animals (Basel). 2019 Nov 11;9(11):952. doi: 10.3390/ani9110952.
Understanding the genetic background underlying the expression of behavioral traits has the potential to fasten the genetic progress for reduced aggressive behavior of pigs. The monoamine oxidase A () gene is known as the "warrior" gene, as it has been previously linked to aggressive behavior in humans and livestock animals. To identify single nucleotide polymorphisms in porcine gene associated with aggressive behavior of pigs, a total of 500 weaned pigs were selected and mixed in 51 pens. In each pen, two aggressive and two docile pigs (a total of 204 pigs) were selected based on their composite aggressive score (CAS). Ear tissue was sampled to extract genomic DNA. Constructs containing variable lengths of truncated porcine promoter were used to determine the promoter activity by a dual luciferase reporter system. The core promoter region was located at -679 bp to -400 bp. A total of nine single nucleotide polymorphisms (SNPs) in gene were genotyped, of which six SNPs had significant differences ( < 0.05) in allele frequency between the aggressive and docile pigs. Linkage disequilibrium and association analyses showed that the pigs inherited the wild genotypes showed more aggressive behavior ( < 0.05) than pigs with the mutant genotypes of the four linked SNPs, rs321936011, rs331624976, rs346245147, and rs346324437. In addition, pigs of GCAA haplotype were more ( < 0.05) aggressive than the pigs with GCGA or ATGG haplotype. The construct containing the wild genotype GG of rs321936011 had lower ( = 0.031) promoter activity compared to the mutant genotype AA. These results suggest that the four linked SNPs in gene could be considered as a molecular marker for behavioral trait selection in pigs.
了解行为性状表达背后的遗传背景，有可能加快猪攻击性行为减少的遗传进展。单胺氧化酶A（）基因被称为“战士”基因，因为它之前已与人及家畜的攻击性行为相关联。为了鉴定猪基因中与猪攻击性行为相关的单核苷酸多态性，总共挑选了500头断奶仔猪并混养在51个猪栏中。根据综合攻击得分（CAS），在每个猪栏中挑选出两头攻击性强的猪和两头温顺的猪（共204头猪）。采集耳部组织以提取基因组DNA。使用包含不同长度截短的猪启动子的构建体，通过双荧光素酶报告系统来确定启动子活性。核心启动子区域位于 -679 bp至 -400 bp。对基因中的总共9个单核苷酸多态性（SNP）进行基因分型，其中6个SNP在攻击性猪和温顺猪之间的等位基因频率存在显著差异（<0.05）。连锁不平衡和关联分析表明，与四个连锁SNP（rs321936011、rs331624976、rs346245147和rs346324437）的突变基因型猪相比，继承野生基因型的猪表现出更强的攻击性行为（<0.05）。此外，GCAA单倍型的猪比GCGA或ATGG单倍型的猪更具攻击性（<0.05）。与rs321936011的突变基因型AA相比，包含野生基因型GG的构建体具有较低的（=0.031）启动子活性。这些结果表明，基因中的四个连锁SNP可被视为猪行为性状选择的分子标记。
