School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, People's Republic of China.
Immunogenetics. 2011 Mar;63(3):155-66. doi: 10.1007/s00251-010-0498-0. Epub 2010 Dec 4.
Major histocompatibility complex (MHC) molecules play an important role in the susceptibility and/or resistance to many diseases. To gain an insight into the MHC background and to facilitate the experimental use of cynomolgus macaques, the second exon of the MhcMafa-DOB, -DPB1, and -DQB1 genes from 143 cynomolgus macaques were characterized by cloning to sequencing. A total of 16 Mafa-DOB, 16 Mafa-DPB1, and 34 Mafa-DQB1 alleles were identified, which revealed limited, moderate, and marked allelic polymorphism at DOB, DPB1, and DQB1, respectively, in a cohort of cynomolgus macaques of Vietnamese origin. In addition, 16 Mafa-DOB, 5 Mafa-DPB1, and 8 Mafa-DQB1 alleles represented novel sequences that had not been reported in earlier studies. Almost of the sequences detected at the DOB and DQB1 locus in the present study belonged to DOB01 (100%) and DQB106 (62%) lineages, respectively. Interestingly, four, three, and one high-frequency alleles were detected at Mafa-DOB, -DPB1, and -DQB1, respectively, in this monkeys. The alleles with the highest frequency among these monkeys were Mafa-DOB010102, Mafa-DPB113, and Mafa-DQB1*0616, and these were found in 33 (25.6%) of 129 monkeys, 32 (31.37%) of 102 monkeys, and 30 (31%) of 143 monkeys, respectively. The high-frequency alleles may represent high priority targets for additional characterization of immune function. We also carried out evolutionary and population analyses using these sequences to reveal population-specific alleles. This information will not only promote the understanding of MHC diversity and polymorphism in the cynomolgus macaque but will also increase the value of this species as a model for biomedical research.
主要组织相容性复合体（MHC）分子在许多疾病的易感性和/或抗性中起着重要作用。为了深入了解 MHC 背景，并促进食蟹猴的实验应用，我们通过克隆测序对来自 143 只食蟹猴的 MhcMafa-DOB、-DPB1 和-DQB1 基因的第二外显子进行了特征描述。总共鉴定出 16 个 Mafa-DOB、16 个 Mafa-DPB1 和 34 个 Mafa-DQB1 等位基因，这表明来自越南起源的食蟹猴群体在 DOB、DPB1 和 DQB1 上分别具有有限、中度和明显的等位基因多态性。此外，16 个 Mafa-DOB、5 个 Mafa-DPB1 和 8 个 Mafa-DQB1 等位基因代表了在早期研究中未报道过的新序列。本研究中在 DOB 和 DQB1 基因座检测到的几乎所有序列分别属于 DOB01（100%）和 DQB106（62%）谱系。有趣的是，在 Mafa-DOB、-DPB1 和-DQB1 中分别检测到四个、三个和一个高频等位基因。在这些猴子中，这些等位基因的最高频率分别为 Mafa-DOB010102、Mafa-DPB113 和 Mafa-DQB1*0616，在 129 只猴子中的 33 只（25.6%）、102 只猴子中的 32 只（31.37%）和 143 只猴子中的 30 只（31%）中发现了这些等位基因。这些高频等位基因可能代表免疫功能进一步特征描述的高优先级目标。我们还使用这些序列进行了进化和群体分析，以揭示群体特异性等位基因。这些信息不仅将促进对食蟹猴 MHC 多样性和多态性的理解，还将增加该物种作为生物医学研究模型的价值。
