数据表明所开发的生长激素1（GH1）基因检测方法在检测加工后的鲑鱼产品时对大西洋鲑具有高度特异性的适用性。
Data representing applicability of developed growth hormone 1 (GH1) gene detection method for detecting Atlantic salmon () at high specificity to processed salmon commodities.
作者信息
Soga Keisuke, Nakamura Kosuke, Ishigaki Takumi, Kimata Shinya, Ohmori Kiyomi, Kishine Masahiro, Mano Junichi, Takabatake Reona, Kitta Kazumi, Nagoya Hiroyuki, Kondo Kazunari
机构信息
National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, 215-9501, Japan.
Chemistry Division, Kanagawa Prefectural Institute of Public Health, 1-3-1 Shimomachiya, Chigasaki, Kanagawa, 253-0087, Japan.
出版信息
Data Brief. 2019 Oct 22;27:104695. doi: 10.1016/j.dib.2019.104695. eCollection 2019 Dec.
This article is referred to the research article entitled "Development of a novel method for specific detection of genetically modified Atlantic salmon, AquAdvantage, using real-time polymerase chain reaction" by Soga et al. (2020). Applicability of the developed (GH1) and (18S rDNA) detection methods using real-time polymerase chain reaction (PCR) for detecting Atlantic salmon () to processed food commodities was examined. DNAs extracted and purified from 24 commodities labelled to include salmon as an ingredient were used as template. Yield and purity of DNAs obtained and Cq values from real-time PCR analyses were provided.
本文引用了Soga等人（2020年）发表的题为“利用实时聚合酶链反应特异性检测转基因大西洋鲑鱼（AquAdvantage）的新方法的开发”的研究文章。研究了利用实时聚合酶链反应（PCR）开发的生长激素1（GH1）和18S核糖体DNA（18S rDNA）检测方法在检测加工食品中的大西洋鲑鱼（Salmo salar）时的适用性。从24种标明含有鲑鱼成分的商品中提取并纯化的DNA用作模板。给出了所获得DNA的产量和纯度以及实时PCR分析的Cq值。
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