Haggis G H, Pawley J B
Canada Department of Agriculture, Ottawa, Ontario.
J Microsc. 1988 Jun;150(Pt 3):211-8. doi: 10.1111/j.1365-2818.1988.tb04639.x.
Triton-extracted, freeze-fractured 3T3 cells have been examined in the Hitachi S-900 field-emission SEM, after light platinum coating, at low beam voltage to evaluate the performance of the microscope under these conditions. For unstained material fixed in glutaraldehyde alone, high-resolution images can be obtained, at accelerating voltages of 1.5-5kV, after rotary deposition of platinum to an average thickness of 1.5-3 nm. Comparisons are made between these results and those of studies by TEM of deep-etch replicas of similar material previously published.
已在日立S - 900场发射扫描电子显微镜中对经Triton提取、冷冻断裂的3T3细胞进行了检查，在进行轻铂镀膜后，于低束电压下评估显微镜在这些条件下的性能。对于仅用戊二醛固定的未染色材料，在1.5 - 5kV的加速电压下，通过旋转沉积铂至平均厚度为1.5 - 3nm后，可获得高分辨率图像。将这些结果与先前发表的关于类似材料深度蚀刻复制品的透射电子显微镜研究结果进行了比较。
