Haggis G H, Bond E F
J Microsc. 1979 Apr;115(3):225-34. doi: 10.1111/j.1365-2818.1979.tb00174.x.
The freeze-fracture thaw-fix (FfTF) technique described in earlier papers is applied in the present work to more detailed study of the chicken erythrocyte, by transmission replicas and high resolution scanning electron microscopy (3 nm scan beam size). The three-dimensional structure of the chromatin, and possibly the non-histone protein matrix, of fractured nuclei is to a large extent retained in this method of preparation and seen in stereomicrographs. In these micrographs the helical sub-structure of the 25 nm chromatin strands can be seen at about the same resolution as that of previously published micrographs in which extracted chromatin is viewed by negative contrast or after metal shadowing. The useful resolution of the secondary electron micrographs, for a suitably mounted specimen, is shown to be as good as that of transmission micrographs of platinum-carbon replicas of the same material.
在本研究中，我们应用了早期论文中描述的冷冻断裂解冻固定（FfTF）技术，通过透射复制品和高分辨率扫描电子显微镜（3纳米扫描束尺寸）对鸡红细胞进行更详细的研究。在这种制备方法中，断裂细胞核的染色质三维结构，可能还有非组蛋白蛋白质基质，在很大程度上得以保留，并在立体显微镜照片中可见。在这些显微照片中，可以看到25纳米染色质链的螺旋亚结构，其分辨率与之前发表的显微照片大致相同，在之前的照片中，提取的染色质通过负染色或金属阴影观察。对于适当安装的标本，二次电子显微照片的有效分辨率显示与相同材料的铂 - 碳复制品的透射显微照片一样好。
