多头绒泡菌肌动蛋白基因的结构与表达

Structure and expression of an actin gene of Physarum polycephalum.

作者信息

Gonzalez-y-Merchand J A, Cox R A

机构信息

Laboratory of Developmental Biochemistry, National Institute for Medical Research, Mill Hill, London, U.K.

出版信息

J Mol Biol. 1988 Jul 5;202(1):161-8. doi: 10.1016/0022-2836(88)90528-1.

DOI:10.1016/0022-2836(88)90528-1

PMID:3172209

Abstract

Physarum polycephalum (strain M3CVIII) contains four unlinked actin gene loci, each with two alleles (ardA1, ardA2, ardB1, ardB2, ardC1, ardC2, ardD1 and ardD2). The 4800 base HindIII fragment of the ardC2 allele was previously isolated as a recombinant phage lambda. We now report the structure of the actin gene sequences (C-actin gene). The gene, which contains four intervening sequences, codes for the principal actin isotype of plasmodia and it is expressed in both the haploid myxamoebal and diploid plasmodial phases of the life cycle. The C-actin isotype is closely related to actins of Dictyostelium, Acanthamoebae, Drosophila, sea urchin and mammalian cytoplasmic actin, and more distantly related to actins of yeast, Entamoebae and Tetrahymena. The ardC1 and ardC2 alleles differ by a 700(+/- 100) base-pair insertion/deletion in the vicinity of the 3' end of the transcribed region of the gene.

摘要

多头绒泡菌（菌株M3CVIII）含有四个不连锁的肌动蛋白基因座，每个基因座有两个等位基因（ardA1、ardA2、ardB1、ardB2、ardC1、ardC2、ardD1和ardD2）。ardC2等位基因的4800个碱基的HindIII片段先前作为重组噬菌体λ被分离出来。我们现在报告肌动蛋白基因序列（C-肌动蛋白基因）的结构。该基因含有四个间隔序列，编码原质团的主要肌动蛋白同型，并且在生命周期的单倍体变形虫和二倍体原质团阶段均有表达。C-肌动蛋白同型与盘基网柄菌、棘阿米巴、果蝇、海胆和哺乳动物细胞质肌动蛋白密切相关，与酵母、内阿米巴和四膜虫的肌动蛋白关系较远。ardC1和ardC2等位基因在基因转录区域3'端附近相差700（±100）个碱基对的插入/缺失。