Dibb N J, Newman A J
Laboratory of Molecular Biology, Medical Research Council Centre, Cambridge, UK.
EMBO J. 1989 Jul;8(7):2015-21. doi: 10.1002/j.1460-2075.1989.tb03609.x.
The unexpected discovery of introns raised many questions about gene evolution. We provide evidence that actin and tubulin introns were gained between the G and R of the conserved coding sequence C/AAGR that is known to flank introns in general and which we call a proto-splice site. We conclude that the tubulin and actin introns are less ancient than the coding sequence and so could not have been involved in the primary evolution of the tubulin and actin genes.
内含子的意外发现引发了许多关于基因进化的问题。我们提供的证据表明,肌动蛋白和微管蛋白内含子是在保守编码序列C/AAGR的G和R之间获得的,已知该保守编码序列通常位于内含子两侧,我们将其称为原剪接位点。我们得出结论,微管蛋白和肌动蛋白内含子的年代比编码序列要近,因此不可能参与微管蛋白和肌动蛋白基因的最初进化。