Sivas Cumhuriyet University Faculty of Veterinary Medicine, Department of Veterinary Virology, 58140, Sivas, Turkey.
Erciyes University Faculty of Veterinary Medicine, Department of Veterinary Virology, 38280, Kayseri, Turkey.
Virus Res. 2020 Jan 15;276:197801. doi: 10.1016/j.virusres.2019.197801. Epub 2019 Nov 10.
Malignant Catarrhal Fever (MCF) is a generalized, definitive lethal disease affecting the epithelial and lymphoid tissues of the respiratory and digestive tract, mainly cattle and some wild ruminants such as deer, buffalo or antelope. The sheep-related form of MCF is known to be present in Turkey and is caused by ovine herpesvirus 2 (OvHV-2). The aim of this study was to reveal the genetic diversity of OvHV-2 strains obtained from MCF cases in Eastern Turkey where the livestock industry has an important impact on economic activities. For this purpose, RTA (Replication and transcription activator), FGARAT (formylglycineamide ribotide amidotransferase) and some of glycoprotein genes (Ov7, Ov8 ex2, ORF27 and Ov9.5) were investigated in blood samples from 24 cattles, clinically diagnosed with MCF. Genomic data of chosen samples were furthermore used to characterize and undergo combined phylogenetic analysis to determine possible alleles and subvariants. The results showed that high level of OvHV-2 diversity existed in selected genes and strains carrying allelic variants might circulate both in two geographically distinct regions and in a region itself. Moreover, three different OvHV-2 types and various subtypes were identified based on multi locus approach. This study provides important data to epidemiological research and thereby helps to determine the source of the virus and understand the spread of the disease.
恶性卡他热（MCF）是一种影响呼吸道和消化道上皮组织和淋巴组织的全身性、明确致死性疾病，主要感染牛和一些野生反刍动物，如鹿、水牛或羚羊。已知绵羊相关形式的 MCF 存在于土耳其，由绵羊疱疹病毒 2（OvHV-2）引起。本研究旨在揭示在畜牧业对经济活动有重要影响的土耳其东部 MCF 病例中获得的 OvHV-2 株的遗传多样性。为此，在 24 头临床上诊断为 MCF 的牛的血液样本中研究了 RTA（复制和转录激活物）、FGARAT（甲酰甘氨酸酰胺核糖基转移酶）和一些糖蛋白基因（Ov7、Ov8 ex2、ORF27 和 Ov9.5）。选择样本的基因组数据进一步用于特征描述和联合系统发育分析，以确定可能的等位基因和亚型。结果表明，所选基因中存在高水平的 OvHV-2 多样性，携带等位基因变异的菌株可能在两个地理上不同的地区以及一个地区内循环。此外，基于多点方法确定了三种不同的 OvHV-2 类型和各种亚型。本研究为流行病学研究提供了重要数据，有助于确定病毒的来源和了解疾病的传播。
