Abd El Rahman Sahar, Ateya Ahmed, El-Beskawy Mohamed, Wernike Kerstin, Hoffmann Bernd, Eschbaumer Michael
Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Department of Animal Husbandry and Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Vet Sci. 2020 Dec 11;7(4):201. doi: 10.3390/vetsci7040201.
Ovine gammaherpesvirus-2 (OvHV-2) causes a lethal disease in cattle and some wild ruminants called malignant catarrhal fever (MCF), which affects the epithelial and lymphoid tissues of the respiratory and digestive tracts and has an important impact on the livestock industry. In this study, MCF was diagnosed in 18 of 427 cattle from different sites in Egypt by its typical clinical signs, found in all 18 animals: corneal opacity, fever, erosions in the buccal cavity, lymphadenitis, and purulent nasal discharge. All affected cattle had been reared in contact with clinically inconspicuous sheep. Of the 18 clinically ill cattle, 13 succumbed to the disease, resulting in estimated morbidity and case fatality rates of 4.2% and 72.2%, respectively. Five samples collected from the affected cattle were positive for OvHV-2 by real-time PCR and were used for sequencing of an 832-bp fragment of the ORF27/gp48 gene. The ORF27 nucleotide sequence of all Egyptian samples was identical, but distinct from viruses found in other parts of Africa and the Mediterranean.
绵羊γ疱疹病毒2型（OvHV - 2）可在牛和一些野生反刍动物中引发一种致命疾病，称为恶性卡他热（MCF），该病会影响呼吸道和消化道的上皮组织及淋巴组织，对畜牧业产生重大影响。在本研究中，通过典型临床症状，在埃及不同地点的427头牛中有18头被诊断为患有MCF，这18头牛均出现了如下症状：角膜混浊、发热、口腔糜烂、淋巴结炎和脓性鼻分泌物。所有患病牛均与外表无明显症状的绵羊接触饲养。在这18头临床患病牛中，13头死于该病，估计发病率和病死率分别为4.2%和72.2%。从患病牛采集的5份样本经实时PCR检测，OvHV - 2呈阳性，并用于对ORF27/gp48基因的832bp片段进行测序。所有埃及样本的ORF27核苷酸序列均相同，但与在非洲其他地区和地中海地区发现的病毒不同。
