Bologa L, Cole R, Chiappelli F, Saneto R P, de Vellis J
Mental Retardation Research Center, UCLA School of Medicine.
J Neurosci Res. 1988;20(2):182-8. doi: 10.1002/jnr.490200206.
An important stage in oligodendrocyte development is the expression of galactocerebroside (GC), the major glycolipid in myelin. Although oligodendrocyte cell lineage and differentiation in vitro have been the object of many studies, to date there is sparse information on the regulation of GC expression in oligodendrocytes already committed to be positive for GC. We report here that GC expression in these cells is controlled by three serum factors. Two of these, possibly a lipoprotein and a mucoprotein, increase GC levels, whereas the third, probably a glycoprotein, exerts an inhibitory effect. The developmental increase of GC in postnatal rat brain cerebral cultures and its induction by serum factors are reversible phenomena. The isolation of the GC-regulatory factors would allow experimental manipulation of impaired GC expression by differentiated oligodendrocytes.
少突胶质细胞发育的一个重要阶段是半乳糖脑苷脂（GC）的表达，它是髓鞘中的主要糖脂。尽管少突胶质细胞谱系及其体外分化一直是许多研究的对象，但迄今为止，关于已确定GC呈阳性的少突胶质细胞中GC表达调控的信息却很少。我们在此报告，这些细胞中GC的表达受三种血清因子控制。其中两种，可能是一种脂蛋白和一种粘蛋白，可提高GC水平，而第三种，可能是一种糖蛋白，则发挥抑制作用。出生后大鼠脑皮质培养物中GC的发育性增加及其受血清因子的诱导是可逆现象。分离GC调节因子将有助于对分化的少突胶质细胞中受损的GC表达进行实验性调控。
