Neurobiology Section, Laboratory of Organ and System Pathophysiology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Roma, Italy.
Cytotechnology. 1991 Feb;5(Suppl 1):158-61. doi: 10.1007/BF00736837.
The aim of the present study was to understand the role of cellular interactions in the choice of the oligodendroglial differentiation route by bipotential progenitors (O-2A progenitors) of oligodendrocytes (OL) and type-2 astrocytes (AS). Cell populations enriched in 0-2A progenitors, obtained from mixed rat cortical glial cultures, were subcultured at low density in BME+10% fetal calf serum on a poly-L-lysine (PLL) substrate (controls) or on a substrate of purified type-1 AS killed by air drying (K-AS), in order to study the interactions between the two cell types independently of the soluble mitogen(s) secreted by type-1 AS. While on PLL the progenitors differentiated into type-2 AS within a week, on K-AS many of them differentiated into OL. However, on K-AS O-2A progenitors proliferated more than on PLL, and this proliferation appeared to be related to unknown polypeptide components of the extracellular matrix produced by type-1 AS. The preferential differentiation of O-2A progenitors into OL on K-AS may thus be partly related to the higher density achieved by the cells on this substrate. This hypothesis is supported by the observation that purified O-2A progenitors subcultured at high density on PLL largely differentiated into OL. The effect of cell density on lineage decision appeared to be related to the secretion of high molecular weight autocrine differentiation factors by O-2A lineage cells.
本研究的目的是了解细胞间相互作用在少突胶质细胞（OL）和星形胶质细胞 2 型（AS）双潜能前体细胞（O-2A 前体细胞）选择少突胶质分化途径中的作用。从混合大鼠皮质神经胶质培养物中获得富含 O-2A 前体细胞的细胞群体，在 BME+10%胎牛血清中于聚-L-赖氨酸（PLL）基底（对照组）或经空气干燥杀死的纯化 1 型 AS 的基底（K-AS）上进行低密度亚培养，以研究两种细胞类型之间的相互作用，而不考虑 1 型 AS 分泌的可溶性有丝分裂原。在前体细胞在 PLL 上分化为 2 型 AS 的 1 周内，在 K-AS 上，其中许多前体细胞分化为 OL。然而，在 K-AS 上，O-2A 前体细胞的增殖多于 PLL，这种增殖似乎与 1 型 AS 产生的细胞外基质的未知多肽成分有关。因此，O-2A 前体细胞在 K-AS 上优先分化为 OL 可能部分与细胞在该基质上达到的更高密度有关。这一假说得到了以下观察结果的支持：在 PLL 上进行高密度亚培养的纯化 O-2A 前体细胞主要分化为 OL。细胞密度对谱系决定的影响似乎与 O-2A 谱系细胞分泌高分子量自分泌分化因子有关。
