Rihs F, Kesselring J, Meier C
Neurologische Universitätsklinik, Inselspital, Bern, Switzerland.
Acta Neuropathol. 1989;78(3):317-24. doi: 10.1007/BF00687762.
Human oligodendrocytes have been successfully maintained in cell cultures for 14 weeks using a modification of a method used previously for animal brain cell cultures. Dissociated cell cultures from spinal cords of human foetuses of 10 to 20 weeks gestional age were investigated for up to 98 days. Oligodendrocytes were identified by monoclonal human antiserum specific for myelin-associated glycoprotein, by polyclonal rabbit antiserum against myelin basic protein, and by the mouse monoclonal antibody I6G1. Astrocytes were identified by polyclonal antibodies against glial fibrillary acidic protein. Immunocytochemical cell identification was corroborated by electron microscopy, by which glial cells were investigated both in situ and in culture. Immunocytochemical staining of myelin-associated glycoprotein showed specifically labelled oligodendrocytes on electron microscopy. The present study indicates that human oligodendrocytes, a putative target in demyelinating disease, can be studied in dissociated cell culture of human foetal spinal cord for several weeks in vitro under stable conditions.
通过对先前用于动物脑细胞培养的方法进行改良，人类少突胶质细胞已在细胞培养中成功维持了14周。对孕龄10至20周的人类胎儿脊髓的解离细胞培养物进行了长达98天的研究。少突胶质细胞通过针对髓鞘相关糖蛋白的单克隆人抗血清、针对髓鞘碱性蛋白的多克隆兔抗血清以及小鼠单克隆抗体I6G1进行鉴定。星形胶质细胞通过针对胶质纤维酸性蛋白的多克隆抗体进行鉴定。免疫细胞化学细胞鉴定通过电子显微镜得到证实，利用电子显微镜对神经胶质细胞进行原位和培养研究。髓鞘相关糖蛋白的免疫细胞化学染色在电子显微镜下显示出特异性标记的少突胶质细胞。本研究表明，人类少突胶质细胞作为脱髓鞘疾病的一个假定靶点，可以在人类胎儿脊髓的解离细胞培养中在体外稳定条件下进行数周的研究。
