一个古老的鼠源内源性逆转录病毒样DNA家族的结构、分布及表达
Structure, distribution, and expression of an ancient murine endogenous retroviruslike DNA family.
作者信息
Obata M M, Khan A S
机构信息
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
出版信息
J Virol. 1988 Nov;62(11):4381-6. doi: 10.1128/JVI.62.11.4381-4386.1988.
An endogenous retroviruslike DNA, B-26, was cloned from a BALB/c mouse embryo gene library by using a generalized murine leukemia virus DNA probe. Southern blot hybridization and nucleotide sequence analyses indicated that B-26 DNA might be a novel member of the GLN DNA family (A. Itin and E. Keshet, J. Virol. 59:301-307, 1986) which contains murine leukemia virus-related pol and env sequences. Northern analysis indicated that B-26-related RNAs of 8.4 and 3.0 kilobases were transcribed in thymus, spleen, brain, and liver tissues of 6-week-old BALB/c mice.
通过使用通用的鼠白血病病毒DNA探针，从BALB/c小鼠胚胎基因文库中克隆出一种内源性类逆转录病毒DNA，即B-26。Southern印迹杂交和核苷酸序列分析表明，B-26 DNA可能是GLN DNA家族（A. Itin和E. Keshet，《病毒学杂志》59:301 - 307，1986）的一个新成员，该家族包含与鼠白血病病毒相关的pol和env序列。Northern分析表明，在6周龄BALB/c小鼠的胸腺、脾脏、大脑和肝脏组织中转录出了8.4和3.0千碱基的B-26相关RNA。
Zotero 插件