Courtney M G, Elder P K, Steffen D L, Getz M J
J Virol. 1982 Aug;43(2):511-8. doi: 10.1128/JVI.43.2.511-518.1982.
The VL30 sequences of mouse DNA are a family of sequences with retrovirus-like structure which code for a 30S RNA transcript that can be packaged into the virions of murine leukemia viruses and thereby transmitted from cell to cell. A Southern blot analysis of these sequences revealed that multiple copies are present in the DNA of all mice examined, regardless of species or geographic origin. Considerable locus polymorphism was also apparent, and at least one of these polymorphisms appeared to reflect the differing chromosomal location of a complete VL30 sequence. These data indicated that VL30 elements are not recent additions to the mouse genome and suggested that the evolution of the VL30 multigene family has been accompanied by duplication and dispersion of VL30 sequences to diverse genomic sites. In addition, we reexamined the issue of genetic relatedness between mouse VL30 sequences and a physically similar family of virus-like elements in the rat genome. We found that many, if not all, rat and mouse VL30 loci contain regions of sequence homology. These data suggested that rodent VL30 sequences have evolved from a common ancestral sequence.
小鼠DNA的VL30序列是一类具有逆转录病毒样结构的序列，它们编码一种30S RNA转录本，该转录本可被包装到鼠白血病病毒的病毒粒子中，从而在细胞间传播。对这些序列的Southern印迹分析表明，在所有检测的小鼠DNA中都存在多个拷贝，无论其物种或地理来源如何。明显还存在相当多的基因座多态性，并且这些多态性中至少有一个似乎反映了完整VL30序列的不同染色体位置。这些数据表明VL30元件不是小鼠基因组的近期添加物，并表明VL30多基因家族的进化伴随着VL30序列向不同基因组位点的复制和分散。此外，我们重新审视了小鼠VL30序列与大鼠基因组中物理上相似的病毒样元件家族之间的遗传相关性问题。我们发现，许多（如果不是全部）大鼠和小鼠VL30基因座都包含序列同源区域。这些数据表明啮齿动物的VL30序列是从一个共同的祖先序列进化而来的。
