采用改良的基于肉汤的方法筛选新型亲脂性药物的抗分枝杆菌活性。
Screening of the antimycobacterial activity of novel lipophilic agents by the modified broth based method.
作者信息
Zandhaghighi Mehdi, Ghazvini Kiarash, Meshkat Zahra, Rezaee Seyed Abdolrahim, Derakhshan Mohammad, Soleimanpour Saman, Hadizadeh Farzin
机构信息
Antimicrobial Resistance Research Center & Department of Medical Bacteriology and Virology, Bu-Ali Research Institute & Ghaem University Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
J Clin Tuberc Other Mycobact Dis. 2016 Feb 11;3:1-5. doi: 10.1016/j.jctube.2016.01.001. eCollection 2016 May.
Most of the introduced susceptibility methods of have some disadvantages for screening. Therefore, the selection of susceptibility assay for evaluating candidate agents must be determined case by case. In this study, we evaluated the validity of a modified broth dilution-based assay in comparison to the gold standard proportional method for microbial sensitivity test of new lipophilic compounds candidate as antitubercular agents. The susceptibilities of 114 strains were separately tested against isoniazid and two lipophilic antitubercular agents (derivative of dihydropyridines) by employing the standard proportional method and a modified broth dilution-based assay. The results for isoniazid testing showed 100% concordance for sensitivity, specificity and reproducibility. In the case of microbial sensitivity test of lipophilic compounds, comparison of the results obtained from these two methods indicates a significant superiority of the modified method over the standard method. Considering the other advantages of this modified method, we concluded that this modified broth dilution-based assay could be utilized effectively for the susceptibility testing of new lipophilic compounds candidate as antitubercular agents.
大多数已引入的药敏试验方法在筛选方面都存在一些缺点。因此，必须根据具体情况来确定用于评估候选药物的药敏试验方法。在本研究中，我们将一种改良的基于肉汤稀释法的试验与用于新型亲脂性化合物（作为抗结核药物候选物）微生物敏感性测试的金标准比例法进行比较，评估了该改良试验的有效性。通过采用标准比例法和改良的基于肉汤稀释法的试验，分别对114株菌株针对异烟肼和两种亲脂性抗结核药物（二氢吡啶衍生物）的敏感性进行了测试。异烟肼测试结果显示，在敏感性、特异性和可重复性方面一致性达100%。在亲脂性化合物的微生物敏感性测试中，这两种方法所得结果的比较表明改良方法明显优于标准方法。考虑到该改良方法的其他优点，我们得出结论，这种改良的基于肉汤稀释法的试验可有效地用于新型亲脂性化合物（作为抗结核药物候选物）的药敏试验。
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