Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
Analyst. 2019 Dec 16;145(1):165-171. doi: 10.1039/c9an02020a.
We report here a facile synthesis of ZnS/CdS heterojunction nanoparticles on an indium-tin oxide (ITO) electrode and their application in the ultrasensitive photoelectrochemical detection of Staphylococcus aureus (S. aureus). The ZnS/CdS/ITO electrode was prepared using one-pot electrodeposition in an acidic solution containing ZnCl2, CdCl2 and Na2S2O3. The optimal ZnS/CdS heterojunction nanoparticles with a Zn/Cd atomic ratio of 1 : 1 showed a high photoelectrochemical response to l-cysteine. l-Cysteine-encapsulated liposome (cysteine@liposome) immunonanocapsules were prepared and used as the labels for photoelectrochemical detection of S. aureus. By coupling cysteine@liposome immunonanocapsule labeling with immunomagnetic separation/enrichment and photoelectrochemical analysis using the ZnS/CdS/ITO electrode, sensitive photoelectrochemical detection of S. aureus was achieved. Under optimal conditions, the linear range for photoelectrochemical detection of S. aureus was from 1 to 4000 CFU mL-1. The proposed method was successfully used for photoelectrochemical detection of S. aureus in milk and juice samples.
我们在此报告了一种在铟锡氧化物（ITO）电极上合成 ZnS/CdS 异质结纳米粒子的简便方法，并将其应用于金黄色葡萄球菌（S. aureus）的超灵敏光电化学检测。ZnS/CdS/ITO 电极是通过在含有 ZnCl2、CdCl2 和 Na2S2O3 的酸性溶液中进行一锅电沉积制备的。具有 Zn/Cd 原子比为 1:1 的最佳 ZnS/CdS 异质结纳米粒子对 l-半胱氨酸表现出高光电化学响应。l-半胱氨酸包封脂质体（cysteine@liposome）免疫纳米胶囊被制备并用作 S. aureus 光电化学检测的标记物。通过将 cysteine@liposome 免疫纳米胶囊标记与免疫磁分离/富集以及使用 ZnS/CdS/ITO 电极进行光电化学分析相结合，实现了对 S. aureus 的敏感光电化学检测。在最佳条件下，光电化学检测 S. aureus 的线性范围为 1 至 4000 CFU mL-1。该方法成功用于牛奶和果汁样品中 S. aureus 的光电化学检测。
