Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, United Kingdom.
Cornea. 2020 Feb;39(2):146-154. doi: 10.1097/ICO.0000000000002192.
To investigate the inflammatory response of the ocular surface with different soft contact lens (CL) replacement frequencies and materials.
Twenty soft CL wearers were required to wear 3 lens types: reusable Acuvue 2 (A2), reusable Acuvue Oasys (AO), and daily disposable Acuvue Oasys (AODD), for 1 week in random sequence in 1 eye with the nonlens-wearing eye acting as a control. Three methods were used to assess the subclinical response: tear cytokine evaluation, in vivo confocal microscopy (IVCM), and impression cytology.
Of 13 cytokines investigated, differences were observed only for IL-12p70, which was present in greater concentrations for A2 (interocular difference 8.8 pg/mL, 95% confidence interval 5.5-12.1) and AO (8.9 [5.7-12.1]) compared with AODD (3.7 [0.6-6.8]). For IVCM, corneal presumed dendritic cell density was lower for AODD (interocular difference 1.9 [-0.1 to 3.9] cells/mm) than for both A2 (9.3 [7.2-11.4]) and AO (10.6 [8.6-12.6]). This trend was the same for the other 5 IVCM measures evaluated. The proportion of CD45 cells in the bulbar conjunctiva was lower for AODD (0.6 [-0.3 to 1.5]%) compared with A2 (4.6 [3.7-5.6]) and AO (4.8 [3.9-5.8]). Similar findings were observed for cells in the upper lid margin.
This work has demonstrated for the first time that daily disposable CL wear produces a minimal subclinical inflammatory response compared with no lens wear over 1 week. By contrast, this inflammatory response is upregulated with reusable lenses but appears to be similar between hydrogel and silicone hydrogel materials over this short time frame.
研究不同软式隐形眼镜（CL）更换频率和材料对眼表面的炎症反应。
20 名软式 CL 佩戴者需按随机顺序在 1 只眼佩戴 3 种镜片类型：可重复使用的 Acuvue 2（A2）、可重复使用的 Acuvue Oasys（AO）和日抛型 Acuvue Oasys（AODD），为期 1 周，非镜片佩戴眼作为对照。采用 3 种方法评估亚临床反应：泪液细胞因子评估、活体共聚焦显微镜（IVCM）和印迹细胞学。
在研究的 13 种细胞因子中，只有白细胞介素 12p70的差异有统计学意义，A2（眼间差异 8.8 pg/mL，95%置信区间 5.5-12.1）和 AO（8.9 [5.7-12.1]）中的浓度高于 AODD（3.7 [0.6-6.8]）。对于 IVCM，角膜假定树突状细胞密度 AODD 组（眼间差异 1.9 [-0.1 至 3.9] 个/毫米）低于 A2 组（9.3 [7.2-11.4]）和 AO 组（10.6 [8.6-12.6]）。这一趋势与评估的其他 5 项 IVCM 指标相同。球结膜 CD45 细胞的比例 AODD 组（0.6 [-0.3 至 1.5]%）低于 A2 组（4.6 [3.7-5.6]）和 AO 组（4.8 [3.9-5.8]）。上睑缘的细胞也观察到类似的发现。
这项工作首次证明，与 1 周无镜片佩戴相比，日抛型 CL 佩戴产生的亚临床炎症反应最小。相比之下，这种炎症反应在可重复使用的镜片中被上调，但在这个短时间内，亲水和硅水凝胶材料之间似乎相似。
