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[采用直接光度法(DPM,胆红素计)测定毛细血管血浆中的胆红素以及采用化学法(2,5-二氯苯基重氮法)测定静脉血样本血清中的胆红素]

[Determination of bilirubin in capillary plasma by a direct photometric method (DPM, bilirubinometer) and the chemical determination of bilirubin in the bilirubin determination (2,5-dichlorophenyldiazonium method) in serum of venous blood samples].

作者信息

Schlebusch H, Liappis N, Niesen M

机构信息

Universitäts-Frauenklinik Bonn.

出版信息

Klin Padiatr. 1988 Jul-Aug;200(4):330-4. doi: 10.1055/s-2008-1033730.

DOI:10.1055/s-2008-1033730
PMID:3172673
Abstract

The determination of bilirubin in serum was performed by the 2.5-dichlorphenyldiazonium method (DPD) and in capillary plasma by the direct photometric method (DPM). Both methods showed a good precision and accuracy. The investigation was carried out in 135 samples with a bilirubin concentration up to 25 mg/dl. The comparison of the two methods in 62 samples with a bilirubin concentration up to 10 mg/dl showed a correlation coefficient of r = 0.862 and in 73 samples with a bilirubin concentration between 10 and 25 mg/dl a correlation coefficient of r = 0.893. In 29 cases (21.5%) we found differences between the two methods of 1.5-4.0 mg/dl. Most of them were in the critical higher range. Discussion of the DPD and DPM methods.

摘要

血清胆红素的测定采用2,5-二氯苯基重氮法(DPD),毛细血管血浆胆红素的测定采用直接光度法(DPM)。两种方法均显示出良好的精密度和准确性。对135份胆红素浓度高达25mg/dl的样本进行了研究。对62份胆红素浓度高达10mg/dl的样本进行两种方法的比较,相关系数r = 0.862;对73份胆红素浓度在10至25mg/dl之间的样本进行比较,相关系数r = 0.893。在29例(21.5%)中,我们发现两种方法之间的差异为1.5 - 4.0mg/dl。其中大多数处于临界较高范围。讨论DPD和DPM方法。

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