Shipman R C, Levy J G
Department of Microbiology, University of British Columbia, Vancouver, Canada.
Leuk Res. 1988;12(7):537-43. doi: 10.1016/0145-2126(88)90082-3.
We have previously described the detection and partial characterization of a common myelogenous leukemia-associated antigen (CAMAL), in CGL and ANLL patients. Both polyclonal and monoclonal (CAMAL-1) antibodies have been raised to p70 (CAMAL) and have been shown to react with both p70 and myeloid leukemia cell preparations. p70 (CAMAL) has been shown to be a monomeric protein of Mr 70,000 and pI 7.2 and was also detectable in the myeloid leukemia cell lines HL60, KG1, K562 and U937, but not in the lymphocytic cell lines Molt-4, Hut-78 and CEM by immunoprecipitation from iodinated cell samples. Using [35S] methionine-labeled cell lines and immunoprecipitation, we have demonstrated the constitutive expression of p70 as well as a major component at p58 and a number at lower molecular weights in the myeloid leukemia cell lines HL60, KG1, K562 and U937, but not in the lymphocytic leukemia cell lines Molt-4, Hut-78 and CEM. The implications of these observations are discussed.
我们之前已经描述了在慢性粒细胞白血病（CGL）和急性非淋巴细胞白血病（ANLL）患者中一种常见的髓系白血病相关抗原（CAMAL）的检测及部分特性。针对p70（CAMAL）已经制备了多克隆抗体和单克隆抗体（CAMAL-1），并且已证明它们能与p70和髓系白血病细胞制剂发生反应。p70（CAMAL）已被证明是一种分子量为70,000、等电点为7.2的单体蛋白，通过对碘化细胞样品进行免疫沉淀，在髓系白血病细胞系HL60、KG1、K562和U937中也可检测到，但在淋巴细胞系Molt-4、Hut-78和CEM中未检测到。利用[35S]甲硫氨酸标记的细胞系和免疫沉淀技术，我们已经证明了在髓系白血病细胞系HL60、KG1、K562和U937中p70以及p58的一种主要成分和一些较低分子量成分的组成型表达，但在淋巴细胞白血病细胞系Molt-4、Hut-78和CEM中未检测到。本文讨论了这些观察结果的意义。
