National Facility for Drug Development for Academia, Pharmaceutical and Allied Industries, Centre for Excellence in Nanobio Translational REsearch (CENTRE), Anna University, BIT campus, Tiruchirappalli, Tamilnadu, 620 024, India.
PG and Research Department of Biotechnology & Bioinformatics, Holy Cross College (Autonomous), Bharathidasan University, Tiruchirappalli, Tamilnadu, 620 002, India.
Metab Brain Dis. 2020 Jan;35(1):159-173. doi: 10.1007/s11011-019-00501-5. Epub 2019 Nov 14.
The anticonvulsant potential of aqueous fruit extract of Passiflora caerulea (PCAE) was evaluated in swiss albino mice induced by pilocarpine. The antioxidant activities of PCAE were determined which showed strong antioxidant activity and the polyphenol compounds such as ginsenoside, naringenin, chrysoeriol 8-c-glucoside, luteolin-6-C-glucoside, apigenin-6,8-di-C-β-D-glucopyranoside were profiled through RP-HPLC and UPLC-ESI-MS/MS. Chronic effects of PCAE on pilocarpine (85 mg/kg; i.p)-induced convulsions were evaluated in Swiss adult male albino mice. PCAE at 100 and 200 mg/kg, (p.o.) and diazepam (5 mg/kg, i.p) were administered once daily for 15 days. In Y-maze test, percentage of correct entry by pilocarpine administered animals were significantly lower when compared to control, whereas PCAE at both doses improved the alteration score significantly. Administration of higher dose (200 mg/kg) of PCAE significantly delayed onset of convulsions and decreased duration of clonic convulsions. Association of ROS production during seizure period was further confirmed by histopathological studies revealing loss of normal neuronal cells in hippocampus region. The data obtained showed anticonvulsant activity and improved cognitive function; reduced the oxidative damage and significantly activated the cholinergic neurotransmission in a dose dependent manner similar to diazepam which is evident in the biochemical parameters and histopathological study, suggesting therapeutic potential for epilepsy and neurodegeneration.
百香果水提物的抗惊厥作用在匹罗卡品诱导的瑞士白化小鼠中进行了评价。测定了 PCAE 的抗氧化活性，结果表明其具有很强的抗氧化活性，多酚类化合物如人参皂苷、柚皮素、橙皮素 8-C-葡萄糖苷、木犀草素-6-C-葡萄糖苷、芹菜素-6,8-二-C-β-D-吡喃葡萄糖苷通过 RP-HPLC 和 UPLC-ESI-MS/MS 进行了分析。在瑞士成年雄性白化小鼠中评价了 PCAE 对匹罗卡品（85mg/kg；腹腔注射）诱导的癫痫的慢性作用。PCAE 以 100 和 200mg/kg（po）和地西泮（5mg/kg，腹腔注射）每天给药一次，共 15 天。在 Y 迷宫测试中，与对照组相比，给予匹罗卡品的动物正确进入的百分比明显降低，而 PCAE 在两种剂量下均显著改善了改变评分。较高剂量（200mg/kg）的 PCAE 给药可显著延迟癫痫发作的发作时间并减少阵挛性癫痫发作的持续时间。在癫痫发作期间 ROS 产生的关联进一步通过组织病理学研究得到证实，该研究显示海马区正常神经元细胞丢失。获得的数据表明，PCAE 具有抗惊厥作用和改善认知功能；减少氧化损伤，并以类似于地西泮的方式以剂量依赖的方式显著激活胆碱能神经传递，这在生化参数和组织病理学研究中是明显的，表明其在癫痫和神经退行性变方面具有治疗潜力。
