Hamid Zeeshan, Armirotti Andrea
D3Validation, Fondazione Istituto Italiano di Tecnologia, Genoa, Italy.
Scuola Superiore Sant'Anna, Pisa, Italy.
Methods Mol Biol. 2020;2084:103-117. doi: 10.1007/978-1-0716-0030-6_6.
Ion mobility has become a valuable tool in mass spectrometry-based lipidomics workflows, thanks to its ability to separate ions in the gas phase based on their size and conformation. Over the last years, it was demonstrated that the comparative analysis of ion mobility data has the potential to highlight the presence of low abundance species in untargeted lipidomics. The present chapter illustrates the background of the topic and guides the reader from the sample preparation to the data analysis of an untargeted lipidomics experiment performed using ion mobility.
离子淌度已成为基于质谱的脂质组学工作流程中的一种重要工具，这得益于其能够在气相中根据离子的大小和构象分离离子。在过去几年中，已证明对离子淌度数据进行比较分析有潜力在非靶向脂质组学中突出低丰度物种的存在。本章阐述了该主题的背景，并指导读者从样品制备到使用离子淌度进行的非靶向脂质组学实验的数据分析。
